天然产物制备与分离教学大纲（Syllabus for the preparation and separation of natural products）Syllabus for the preparation and separation of natural products - a syllabus for the preparation and separation of natural productsI. Basic information of the courseCourse Name: preparation and separation of natural products Preparation and Isolation of Natural ProductsCourse Code: 20403440Course type: required courseClass: 112 Credits: 7Two, the purpose and requirements of teachingThis experiment is the basis of learning the students through organic chemistry, botany, biochemistry and natural medicine chemistry courses, further training of basic experimental skills, to enable students to master the commonly used extraction, separation and identification of natural products physicochemical method and means of understanding and, to lay the foundation for the study and the work deep in the future.Students are required to improve their practical ability and ability to analyze and solve problems through the study of this experiment course.Three, teaching contentExperiment 1. Preliminary test of chemical composition of natural medicine (2 hours)Experiment purpose and requirement1, to understand the significance of natural medicine chemical composition pre-test.2. Master the procedures and methods of the preliminary test of the chemical composition of natural drugs3, learning to make preliminary judgments through the results of the pre-test.Experiment two extraction, isolation and identification of diosgenin from Dioscorea zingiberensis (8 hours)Experiment purpose and requirement1. Mastering the extraction method of steroidal saponin.2. Familiar with the properties and identification methods of diosgenin.Experiment three. Extraction, isolation and identification of rutin (8 hours)Experiment purpose and requirement1. Through the extraction and purification of rutin, the principle and operation of flavonoids extraction by alkali soluble acid precipitation method were mastered.2. Master the method of flavone glycoside hydrolysis to prepare flavone aglycone.3. Understanding the general properties of flavonoids.4. Understand the application of UV spectrum in the determination of flavonoids structure.Experiment four extraction, isolation and identification of psoralen and psoralen (10 hours)Experiment purpose and requirement1, grasp the solvent extraction of furan coumarin operation technology.2. Through the separation of psoralen and psoralen, familiar with the dry column chromatography operation technology.3. Grasp the identification methods of coumarin compounds.Experiment five extraction and separation of oleanolic acid (8 hours)Experiment purpose and requirement1. Master the extraction, isolation and identification technology of three triterpenoid saponins, and be familiar with the properties of three terpene saponins.2. Grasp the principle and operation of two-phase solvent hydrolysis.Experiment six systematic separation of alkaloids (8 hours)Experiment purpose and requirement1, master the systematic separation of alkaloids in each step of the specific operation.2. Understanding the principle and application of alkaloids system separation.Experiment seven. Extraction, isolation and identification of alkaloids from Chinese herbal medicine (12 hours)Experiment purpose and requirement1. Master the extraction of total alkaloids and the principle and method of separation and purification of liposoluble alkaloids and water-soluble alkaloids, phenolic tertiary amine bases and non phenolic tertiary amine bases, water-soluble alkaloids and water-soluble impurities.2. Study the separation of alkaloids by adsorption column chromatography, and master the general column chromatography operation method.3, grasp the commonly used methods of identification of alkaloids.Experiment eight separation of plant natural pigment protein complex and separation of green gel electrophoresis (12 hours)Experiment purpose and requirement1, master the solubility of pigment protein complexes and the use of detergents to increase separation membrane protein.2, grasp the principle of electrophoresis and practical operation.Experiment nine separation of chloroplast and chloroplast components by differential centrifugation and density gradient centrifugation (14 hours)Experiment purpose and requirement1, master the commonly used methods of cell fragmentation in laboratory and the characteristics of various methods.2, master the commonly used centrifugal technology and application.3. Mastering the preparation of discontinuous gradients.Experiment ten extraction and spectral analysis of plant natural pigment (4 hours)Experiment purpose and requirement1. Grasp the method of pigment extraction and the method of pigment content determination.2. Grasp the p