分类号： _ 单位代码： 10335 密 级： _ 学 号： 10716093 博博 士士 学学 位位 论论 文文 番茄中的两个番茄中的两个 MAPKs 和拟南芥中的一个和拟南芥中的一个 MAPKKK 在抗性在抗性 反应调控中的功能分析反应调控中的功能分析 Functional analysis of tomato MAPKs SlMPK4 and SlMPK6 and an Arabidopsis Raf-like MAPKKK raf43 in defense response 申请人姓名： Nasar-um-Minullah 指导教师： 宋凤鸣 教授 专业名称： 植物病理学 所在学院： 农业与生物技术学院 论文提交日期: 2011 年 6 月 中国杭州中国杭州 I 单位代码单位代码(Code)：10335 Student Number：10716093 Functional analysis of Tomato MAPKs SlMPK4 and SlMPK6 and an Arabidopsis Raf-like MAPKKK raf43 in defense response Authors signature: Supervisors signature: Defense Committee Chairperson: Professor Shaojian Zheng Defense Committee Members: Professor Xueping Zhou Professor Guochang Sun Professor Jingwu Zheng Professor Zhonghua Ma Professor Fengming Song Date of oral defense： June 8th, 2011 II Functional analysis of tomato MAPKs SlMPK4 and SlMPK6 and an Arabidopsis Raf-like MAPKKK raf43 in defense response A Dissertation Submitted in Partial Fulfillment of the Requirements for the degree of DOCTOR OF PHILOSOPHY in PLANT PATHOLOGY By Nasar-um-Minullah Zhejiang University Hangzhou, P. R. China June, 2011 III Dissertation for the Degree of Doctor of Philosophy Functional analysis of tomato MAPKs SlMPK4 and SlMPK6 and an Arabidopsis Raf-like MAPKKK raf43 in defense response By Nasar-um-Minullah Major: Molecular Plant Pathology Speciality: Functional Genomics Supervisor: Professor Fengming Song Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University, Hangzhou P. R. China IV THESIS RELEASE CERTIFICATE The author has agreed that the library, Zhejiang University, Hangzhou, China, can make this thesis freely available for inspection. Moreover, the author has agreed that permission of extensive copying of this thesis for scholarly purpose may be granted by the professor or professors who has supervised the thesis work herein or, in their absence, by the Head of the Institute, or the Dean of faculty in which this work was conducted. It is understood that due recognition will be given to the author of this thesis and the Zhejiang University, Hangzhou in any use of the materials in this thesis. Copying or publication or any use of the thesis for financial gain without the prior approval by the Zhejiang University, Hangzhou and the authors written permission is prohibited. Request for permission to copy or to make any other use of materials in this thesis in whole or in part should be addressed to: The Head of the Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Peoples Republic of China. V Declaration I hereby declare that this dissertation is my original work carried out under the guidance and supervision of Professor Fengming Song, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China and has not been submitted for examination to another University or any other institution of higher learning for the award of a degree. . Nasar-un-Minullah Date Institute of Biotechnology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Peoples Republic of China. VI CERTIFICATION This is to certify that the work presented herein titled “Functional analysis of tomato MAPKs SlMPK4 and SlMPK6 and an Arabidopsis Raf-like MAPKKK raf43 in defense response” was carried out and submitted by Mr. Nasar-um-Minullah, candidate for the degree of Doctor of Philosophy (PhD) in plant pathology, at the College of Agriculture and Biotechnology, Zhejiang University, under my supervision. The investigation was an original work and the research report has not been submitted earlier for the award of any other degree. Supervisor Professor Fengming Song Date . College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, Peoples Republic of China. VII To those who taught me even a single word of knowledge Some sowers never reap. Yet, many reapers take the crop for granted. VIII ACKNOWLEDGEMENTS I am indebted to the Higher Education Commission Pakistan for providing me the scholarship to pursue PhD studies in China. Thanks to China Scholarship Council and International College of Zhejiang University for providing support during my stay. I am grateful to my research supervisor, Prof Fengming Song, for providing me opportunity to conduct my PhD research work in his lab and for his valuable guidance throughout the entire course of my research. His guidance always led me through my research problems and with that help I am able to complete my research. Special thanks to Dr Dayong Li for his help and friendly manner of easing me out of problems. I also want to extend my gratitude to all of my lab fellows, past and present, as we all worked like a team. Thanks to the teachers and students