河北大学 硕士学位论文 磁铁矿矿坑水细菌分离与多样性分析 姓名：安秀芳 申请学位级别：硕士 专业：微生物学 指导教师：吕志堂 2011-06 ? I ? ?2? !“#?$%?+:;?ABCD?:;5 1/5PCA ?*?qB9:0?) 23 Ufi ?CD?%ED?2*?lDFG? ?2?C?*“H%?UZI?fi J*?K?ED?2?C%L2 K1E?MK52009 ?N*Bacelar-Nicolau % Johnson10?O?P?QRS Conwy Valley TU?V?(Cae Coch)4?0%W?VXY?xZ0? ?U u=?“H4lm?H?(Ferrimicrobium acidiphilum gen. nov., sp. nov.) %?x?(Ferrithrix thermotolerans gen. nov., sp. nov.)*“H1?+?2?*?+?)?52000 ? Kelly % Wood Q R 16S rDNA -“fg?$?bM?)?“#?H$o?(Acidithiobacillus ferrooxidans) 145?D?+4?4xZX8?“H*H41 2h?*?a?6VFG?“?*Ktb 15-165?“$H$o? (Acidithiobacillus thiooxidans)14,17 *fi ? pH ? 0.5 ?0*;?$% ?$?2?(H2SO4 )*;N?1?fi B*? +12wx6?M6V?|?18 k“#?H$o?fi “#?% FG$*?1$*?l?D?4$O2?fi B1?*I? $4?%6V$? ?012L1?JKfi Bfl ?19-21 5 ?5fl “H ?“#?(Leptospirillum ferrooxidans)22?H?(Leptospirillum ferriphilum)23 *I?53?fl *H?g?6? ?“6V? *?+?fi %?24-28 5 +?fl ?fl ? ?*l12?T?8? ?x?%?X *IE?6VFG?”?T2l ?no29-315 12x6g“?DMK?2?no* ,g?)? ? “?6?DM%? 6V? 024-27,325 1.2.2? 12?I12?b?1?C?u? ?F Gd?(?d?325 gp“H12+? d?yz0?c?MK?34-38 5 12?b?:fi Mc?2 |%?*K?6VFG?%v?*?*12?b1?% ?FG?+l?Y(?%?35,395?12? ? 1 ? 3 ?fi 3*?12?(?FG Fe ? Mn?Au?Ag?Cu?Pb *?40 ?“H?6?C?k“H?l? #?6? 18kg/t*?(Pseudomonas maltophila)?6V? v?(Staphylococcus aureus)O2)?M? 30%35k?*12? ?y?1?C?* ?%?I?0(O ? ?O2?D6V-?O25 1990 ? Zierenberg R A41?+? ?(Northern Gorda Ridge)xZ?8?0?5 *?I 12?C?(?R/?5,?*12?y?FG?D yc?Dy* l?d?5 Fe % Mn I,3?6V5 Nealson 42 ?+? pH ?N?“?12?%?8O?*?+?O?*?+ ? 4 ?(MV)%?(MS)5?I? 12?0?%?.51979 ?*W?X? Donovan 51“HW? ?AB? Cement CD/E?F0?4?2*?Bb?X83* 89*?4?2?G?V?*?d?C12zO I?52-53 5?H?I?d?Ifi G?J?5 1.2.3? ?8?12?6V?KL454? “?M? *Z b?%1|1?z45?xTa%?a?l59-60 ?u ?%BM6V?4T?bl1?(?X?U* g? ?D?271 5?p?r?$?fi ?1?O2 ? 1 ? 5 CM6V?d?O2)6VFG725 3+?0“Hst? 0+%B?(VI)?73 *?u?v?)?(VI)?M?%?fi J? ?*fi ?jL4? Cr6+?L4? Cr3+*w?T?Ta?8? 12VW74512VWde“b?xyz?Uz?4*?M6 V?U?8?VW?“:5 1.2.4? ?8?g?)? =?F =?GH IJ(%) OTU1cf01,cf02,cf04,cf19, cp2,cp04,cp07,cp19 Brevundimonas OTU2cf25Brevundimonas OTU3cf41, cp03Brevundimonas OTU4cp05Brevundimonas 1225.53 OTU5cp5Bacillus OTU6ce45,cf30Bacillus OTU7cp64Bacillus OTU8cp42Bacillus OTU9cf50,cp57Bacillus OTU10cp4Bacillus 817.02 OTU11cp06,cf29, e37,ce40Sphingomonas OTU12ce29Sphingomonas OTU13ce33Sphingomonas 612.77 OTU14cp53Micrococcus OTU15cp1Micrococcus OTU16cp21Micrococcus OTU17cp38Micrococcus OTU18cp50Micrococcus 510.64 OTU19ce1,ce41Xanthobacter OTU20ce7Xanthobacter 36.38 OTU21cp15Sneathiellaceae1 K OTU22cp26Sneathiellaceae1 K 24.25 OTU23cp45Arthrobacter OTU24ce2Arthrobacter 22.13 OTU25cp02,cp4502Deinococcus24.25 OTU26cp22Microbacterium14.25 OTU27cp3Rhodococcus12.13 OTU28cp28Staphylococcus12.13 OTU29cf26Paracoccus12.13 OTU30cf03Azospirillum12.13 OTU31cp17 pseudomonas12.13 OTU32cf40Rhodobacter12.13 ? !?“#$ 32 cp53 Micrococcus antarcticus NR_025285 cp1 Micrococcus endophyticus EU005372 Micrococcus lylae NR_026200 cp21 cp38 Micrococcus yunnanensis FJ214355 Micrococcus luteus AJ536198 ce2 Zhihengliuella aestuarii EU939716 Arthrobacter sulfonivorans AF235091 cp45 Arthrobacter globiformis X80736 Arthrobacter pascens X80740 Arthrobacter ramosus NR_026193 cp50 Micrococcus flavus DQ491453 Microbacterium arabinogalactanolyticum Y17228 Microbacterium paraoxydans NR_025548 cp22 Microbacterium esteraromaticum AB210977 Rhodococcus erythropolis NR_037024 Rhodococcus yunnanensis AY602219 cp3 Rhodococcus fascians NR_037021 Deinococcus grandis NR_026399 Deinococcus navajonensis AY743259 Deinococcus radiodurans Y11332 Deinococcus wulumuqiensis EU025028 cp02 cp4502 Deinococcus ficus AY941086 Deinococcus mumbaiensis DQ003135 ce45 cf30 Bacillus asahii NR_024817 Bacillus novalis AJ542512 Bacillus niacini NR_024695 Bacillus psychrosaccharolyticus AB021195 Bacillus butanolivorans EF206294 cp5 Bacillus simplex AJ439078 Bacillus muralis AJ316309 cp28 Staphylococcus epidermidis STA16SRR11 cp64 Bacillus subtilis AJ276351 Bacillus stratosphericus AJ831841 Bacillus aerophilus AJ831844 Bacillus altitudinis AJ831842 cp4 cp42 cp57 cf50 Bacillus pumilus AY876289 Brevundimonas nasdae AB071954 Brevundimonas aurantiaca AJ227787 Brevundimonas vesicularis AJ227780 Brevundimonas intermedia AJ227786 cf19,cp19,cf01,cf02,cf04,cp04,cp07,cp2 cf25 Brevundimonas alba AJ227785 Brevundimonas