Chapter8HicropialGroptRandGrow尔CorztrolChapterOutlineMethodsofCulturingMicroorganismsMeasurementofGrowthPopulationGrowthContinuousCuiture:theChemostatEffectofEnvironmentonGrowthPhysicalandChemicalControlofMicrobes一、MethodsofCulturingMicroorganisms。FiveT“s“Inoculation(接种)“Incubation焰养“Isolation(切离/“Inspection(观寨)“Identification(益定)1、Inoculation。Placedintoacontainerofsterilemedium;。Usingasteriletooltospreadthesampleonthesurfaceofasolidmediumortointroducethesampleintoaflaskortube.2、Incubation。Atemperature一controlledchamberorincubator;。Settingtheoptimumtemperatureandgascontent;。Incubationproducesaculture-thevisiblegrowthofthemicrobeinthemediumi.3、Tsolation。MakeapureculturethatcontainsonlyasingleSpeciesofmicrobe:.Separatecolonies(茵蒙onsolidmedia;Furtherisolation-subculturing移种、接种.Meoscopeew(1丿StreakPlate钊(平板划线分离法。Graduallythinsoutthesample;*SeparatesthecellsspatiallyoverseveralsectionsofthePlate.*Becauseofitseffectivenessandeconomyofmaterials,thestreakplateisthemethodofchoiceformostapplication.StepsinaSteakplte55o【分区划线(造用于洛度较大的样品】进续刘线(适用于浸度轻小的样品】ewUniiantaue(2丿SpreadPlate【(涂布法。Tomakea10-fold(10一dilutions;1ml1ml1ml1ml1ml1ml侧岫_帼、0CEE4SampletoM1100410404105105becounted110