ClassifiedIndex：Confidential：noCode：10224No10042llDissertationfortheMasterDegreeTraitsAnalysisofthe“GlossyWaxlessMutant，inCabbageCandidate：MuXiangliSupervisor：ProfWangChaoDegreeCategory：MasterofAgricultureCollege：CollegeofHorticultureFirstleveldiscipline：HorticultureSecondleveldiscipline：01ericultureHarbinChinaJune2013坠一锄燃必|曼曼皇皇_量鼍量量鼍篡皇量曼舅薯|鲁曼曼曼量皇皇曼皇量量皇鲁邕鼍篡曼曼_墨置皇鼍一-VV目录中文摘要I英文摘要1弓I言111研究的目的与意义、研究内容、试验技术路线1111研究的目的与意义1112研究内容1113试验设计112文献综述2121植物叶片表皮蜡质研究进展2122分子标记技术在蔬菜作物上的应用研究概况92材料与方法一l921试验材料19211植物材料19212主要仪器及实验试剂1922试验方法19221结球甘蓝无蜡粉突变体田间性状调查19222结球甘蓝无蜡粉突变体遗传规律研究19223结球甘蓝无蜡粉突变体叶片表皮超微结构观察20224基因组DNA的提取及混合池的建立20225AFLP分子标记203结果与分析2531结球甘蓝无蜡粉突变体田间性状表现2632结球甘蓝无蜡粉突变体遗传规律分析。2733结球甘蓝无蜡粉突变体叶片表皮超微结构观察结果27331一叶苗期叶表皮蜡质超微观察27332三叶苗期叶表皮蜡质超微观察28333结球前期叶表皮蜡质超微观察29334结球后期叶表皮蜡质超微观察30335成熟期叶表皮蜡质超微观察3034结球甘蓝基因组DNA的提取3l35AFLP枥；记32351基因组DNA酶切、连接结果检测32352AFLP预扩增产物的检测32353AFLP选择性扩增体系的优化及AFLP选择性扩增33354AFLP引物筛选。33I东北农业大学农学硕士学位论文355亮叶981030981030(野生)F2代单株PCR扩增36356连锁分析及遗传距离的确定。40357连锁关系作图404讨论4141关于结球甘蓝无蜡粉突变体抗虫性的问题4l42关于结球甘蓝无蜡粉突变体的超微结构问题4l43关于无蜡粉突变性状的遗传特点4l44关于结球甘蓝无蜡粉基因分子标记的遗传距离425结论4351结球甘蓝无蜡粉突变体田间性状表现4352结球甘蓝无蜡粉基因的遗传分析4353结球甘蓝无蜡粉突变体叶片表皮蜡质超微结构观察4354结球甘蓝无蜡粉基因的连锁距离43致谢44参考文献459ff录52攻读硕士学位期间发表的学术论文56IICONTENTSCoNTENTSAbstractinChinese。IAbstractinEnglishIII1Introduction。111Purpose，meaningandcontentofthisstudy1111Purposeandsignificanceofthisstudy1112Contentoftheresearch1113Experimentplanoftheresearch112Summaryofdocument2121Theresearchprogressofcuticularwaxonplantleaves2122Theresearchprogressofmolecularmarkertechniqueanditsapplicationinvegetables92Materialsands1921Experimentalmaterials19211Plantmaterials19212Maininstrumentsandlaboratoryreagents1922Experimentals19221Investigatefieldcharactersofwax-lessmutantofheadcabbage19222Investigatethegeneticlawsofwaxlessmutantofheadcabbage19223Observeultrastructureofthewaxlessmutantepiwaxonheadcabbage20224ExtractegenomeDNAandestablishthemixedpool20225AFLPmolecularmarkers203Resultsandanalysis2531Traitsfieldperanceofwaxlessmutantofheadcabbage2632Theanalysisresultsofthegeneticlawsofwax-lessmutantofheadcabbage2733Observationofuitrastructureofthewax-lessmutantepiWaxoncabbage27331ObservationoftheepiWaxat1。leafstage27332Observationoftheepiwaxat3啊leafstage28333Observationoftheepiwaxatbeforeheadingstage。29334Observationoftheepiwaxatafterheadingstage30335Observationoftheepiwaxatmaturestage3034ExtractionoftotalgenomeDNA一3135AFLPmarkers32351TheresultsofgenomicDNAdigestionandtigation32352Thedetectionoftheproductsofpreamplification32353Theresultsofsystemoptimizationandselectiveamplification33东北农业大学农学硕士学位论文354TheresultsofAFLPprimersscreening