Dynamic Light ScatteringDynamic Light Scattering Measuring Platelet Quality and Measuring Platelet Quality and Bacterial ContaminationBacterial Contamination Elisabeth Maurer, Ph.D.Elisabeth Maurer, Ph.D. Canadian Blood Services, R 21(4):295 8 of 17 Solving the Problem: DLS-PM ScoreSolving the Problem: DLS-PM Score High DLS-PM scoreHigh DLS-PM score Medium Medium DLS-PM scoreDLS-PM score Low DLS-PM scoreLow DLS-PM score Most fresh platelet transfusions Most platelet transfusions until day 7 - 12 of storage Temperature Response Microparticles Platelet Size 9 of 17 The Invention:The Invention: The Dynamic Light Scattering Platelet Monitor (DLS-PM)The Dynamic Light Scattering Platelet Monitor (DLS-PM) Non-invasive Small sample volume Fast Inexpensive Phys Med Biol 2006; 51:3747 10 of 17 The Invention: AccomplishmentsThe Invention: Accomplishments May - Aug 2007 First clinical pilot study 2006 Validation Latex beads Platelets Bacteria Proteins Nanoparticles Sept 2005 First generation instrument April 2005 First prototype Nov 2003 Proof of principle 11 of 17 CoCorrelation of rrelation of D DLS-PM results with LS-PM results with i in n v vivoivo transfusion transfusion o outcomeutcome 49 patients enrolled, 160 transfusions49 patients enrolled, 160 transfusions Location: Vancouver General HospitalLocation: Vancouver General Hospital Data collected:Data collected: DLS-PM scoresDLS-PM scores 1h and 24h patient platelet counts 1h and 24h patient platelet counts transfusion merit transfusion merit The Invention: Clinical ResultsThe Invention: Clinical Results DLS-PM score vs. transfusion merit 12 of 17 104/160 transfusions 65 % Transfusion merit correlates with DLS-PM score The Invention: CoDIVO ResultsThe Invention: CoDIVO Results 13 of 17 INVASIVE No single instrument can determine platelet quality and function in multiple dimensions Impedance Optical Immunofluorescence Hematology analyzers cannot assess bacterial contamination BacT/Alert required Sysmex XE-2100 Kobe Advia 120 Siemens FacsCanto II BD Other Technology: Platelet CountingOther Technology: Platelet Counting BacT/Alert bioMrieux 14 of 17 Sysmex XE2100Sysmex XE2100 Optical platelet count + 1-micron beads Optical platelet count + 1-micron beads Other Technology: AccuracyOther Technology: Accuracy Without beads With beads Beads 15 of 17 Optical methods - Low sensitivity Especially for platelet counts 15,000 / L Other Technology: SensitivityOther Technology: Sensitivity Segal et al. BrJ Haematol 2005; 128:520. 16 of 17 v Mandated technology for hospitals / blood banks v 10,000 hospitals / blood banks in North America v Extended shelf life - reduced outdate-discard rate v Patient safety v Monitor manufacturing processes SummarySummary DLS-PM: The only technology for noninvasive bacterial detection and platelet quality testing at the same time Thank You! Keddie Brown, iCapture Audrey Labrie, CBS Dr. Mark Scott, CBS Dr. Kate Chipperfield, VGH Cheryl Pittendreigh, CBS Nobu Kitamura, CBS CBS Intellectual Property, Intramural and Bridge Funding KeddieKeddie AudreyAudrey NobuNobu CherylCheryl