L因表达与调控沥芒许研究基因转录调控的方法ReportergeneassayEMSA(Electrophoreticmobilityshiftassay)FootpringtingMethylationassayTetoffandTeUonassayNortiernBlottingChPSAGEWesternblottingassayChromatinImmunoprecipitation(CHHIP)一-reaLtimeanalysisforproteiyDNAinteractionYoumustKnoWorhave;1.DNAsequencearoundinterestingbindsites2.Proteinsthatcouldbindtothesites3.AntibodyfortheproteinExample:“RNAPolITattranscriptionstartpoint山口白-NacleicAcidsResearch,2019,32(LD:1.8ObtaintissuesfromanimalorhumianCrosslinkchromatinandbindingproteinsTreattissuesby194formaldehydefor12minandthenstopreactionby0.125MGlycineDisaggregatetissuebyhomogenizepcollectcellsBreakcellsbybuffercontaining0.594NP-40,spintocollectnucleiBreaknncleibybuffercontaining194SDSSonicatechromatininto500bpfragmentsBlockproteinA/GagarosebyXDNA,RNAandBSA+一一a(118.IncubatesonicatedDNAwithblockedproteinA/Gagarosetoremovenon-specificbind,spintogetsupernatant,SavesaImple5038仪刘的TimeaferPH仪)SerialAnalysisofGeneExpression(SAGBE)一一分析特定细胞或组织中的基因表达谱SAGEisbasedonthefoliowingprinciple:9bpDNAtagcontainssufftcientinformationtoumiduelyidentifyaImRNANNNN4“=256NNNNN45=1024NNNNNN45=4096NNNNNNN47=16376NNNNNNNN4=65504NNNNNNNNN49=262019BsmF1:GGGACNNNNNNNNNNNNNNNNNNNNCCCTGNNNNNNNNNNNNNNNNNNNN1NlalitNNNNCATGNNNNNNNNNTAcNNNNNStreptavidin:AproteinproducedbythebacteriumStreptomycesavhasfourbindingsitesforbiotin.Ithasbeenusedextensivelyasprobesinimmunochemicalsystems,conjugatedtoantibodies,enzymesorfluorochromes.比