生物信息学 大作业 参考答案 芍药 ACS 基因的生物信息学分析 姓名 班级 学号 2016 年 4 月 11 日 一 芍药一 芍药 ACS 基因序列及其编码的蛋白的功能基因序列及其编码的蛋白的功能 乙烯是存在于植物体内的唯一的一种气态植物激素 调控植物花 果和叶片的衰老进程 乙烯的合成 主要在转录水平上受到 ACS ACC synthase ACC 合酶 和 ACC 氧化酶的调控 ACS 将 SAM S adenosyl methionine S 腺苷甲硫氨酸 转化为 ACC ACC 氧化酶进一步将 ACC 转化为乙烯 3 分 本作业的研究材料为芍药 ACS ACC synthase 基因 NCBI 序列号为 JX512359 其核苷酸序列及编 码的氨基酸序列如图 1 5 分 acatggggacagcatacgcaataaaagcaataccaaacttcctt 44 taaatctgcttctgctattcgatcatcattgttacattcttctctacaaacctcctctgttttttcttcaaatttctatagtcacataaa 134 ATGGGATTCATGTCCACAGATCAACAAAAGCAATTGCTGTCAAAGATGGCAACAGGCAATGGCCATGGAGAAGACTCTCCTTACTTTGAT 224 M G F M S T D Q Q K Q L L S K M A T G N G H G E D S P Y F D 30 GGTTGGAAGGCATATGACAGCAATCCATTTCATCTTAATAACAATCCTAATGGGGTTATCCAAATGGGACTTGTAGAAAATCTGCTTTCC 314 G W K A Y D S N P F H L N N N P N G V I Q M G L V E N L L S 60 TTTGATGTGATTCAAGAATGGGTTCTGAATAATCCAAAAGCCTCCATTTGCACGCCAGAAGGAATTAATGAATTCAGAGATACTGCTATT 404 F D V I Q E W V L N N P K A S I C T P E G I N E F R D T A I 90 TTTCAGGATTATCATGGGTTTCCAGAGTTCAGAAATGCTATTGCAAAATTTATGGGAAAAGTGGGAGGAGGAAGAGTCACATTCGATCCA 494 F Q D Y H G F P E F R N A I A K F M G K V G G G R V T F D P 120 GACCGCATTGTCATGAGTGGTGGGGCGACTGGAGCTCATGAGATTCTGGCCTTCTGCTTGGCTGACCCTGGCGATGCATTTCTGGTGCCA 584 D R I V M S G G A TG A T G A H E I L A F C L A D P G D A F L V P 150 ACTCCATATTATCCAGGATATGATCGCGATTTGAGATGGCGAACAGGAGCTCAACTGCTTCCCGTTCAATGCGACAGCTCCAACAATTTC 674 T P Y Y Y P G Y D R D L R W R T G A Q L L P V Q C D S S N N F 180 ATGGTTACCATAAGCGCCCTAGAATTGGCATACCAGAAAGCTCAAGATGCAAACATTAAAGTAAAGGGTTTGATCATAAACAACCCATCA 764 M V T I S A L E L A Y Q K A Q D A N I K V K G L I I N N P S 210 AATCCATTAGGCACTGTCTTAAATGGAGAGACACTAAAAACTATAGTGAACTTCATCAATGAAAAGAACATCCACCTTGTTTGTGATGAG 854 N N P L G T V L N G E T L K T I V N F I N E K N I H L V C D E 240 ATTTACGCGGCCACTGTCTTTTGCCAGCCTCGTTTCATTAGCATTGCGGAAATAATAAACGACATGGATGGTGTTAATCGAAATCTCATC 944 I Y Y A A T V F C Q P R F I S I A E I I N D M D G V N R N L I 270 CACATTGTCTACAGTCTCTCAAAGGACATGGGGTTCCCTGGATTTAGGGTTGGCATTGTGTATTCATACAATGATGCCGTAGTCAGTTGT 1034 H I V Y S S L S KS K D M G F P G F R R V G I V Y S Y N D A V V S C 300 GCGCGCAAGATGTCTAGCTTTGGGCTAGTTTCAACTCAAACCCAACACCTAATTGCATCAATGCTATCAGATGAACACTTCACCGAGAGA 1124 A R K M S S F G L V S T Q T Q H L I A S M L S D E H F T E R 330 TATATTGTGGAGAGTGCAAAGAAATTGGCAGAAAGGCAGAGGCGCTTCACTAGGGGACTTGCTCAAGTAGGCATTAATTTTTTGAAGAGC 1214 Y I V E S A K K L A E R Q R R F T R G L A Q V G I N F L K S 360 AATGCAGGTCTCTTCTTATGGATGGATTTAAGCTCGCTGCTCAAGGAGAAGACGGTCGAAGAAGAGCTAACACTTTGGCGAGTGATAATC 1304 N A G L F L W M D L S S L L K E K T V E E E L T L W R V I I 390 AATGAAGTTAAACTCAATGTTTCACCTGGTTCATCTTTTCATTGCTCGGAGCCTGGATGGTTTAGGGTTTGCTTTGCTAACATGGATGAT 1394 N E V K L N V S P G S S F H C S E P G W F R V C F A N M D D 420 GCCACCATGGAAGTTGCTCTTCGAAGGATTCGAACATTTGCACTTAAGGCCAAGGAAGCAGATGTGCCAAGGAAGAAACAAAGTTGGCAA 1484 A T M E V A L R R I R T F A L K A K E A D V P R K K Q S W Q 450 AACAACAACCTTAGATTCAGCTTCAAATCTGGGAAATATGATGATGTCTTGTTGTCTCCTCGTATGATGTCCCCTTGCATGAGGTCCCCT 1574 N N N L R F S F K S G K Y D D V L L S P R M M S P C M R S P 480 CATTCCCCCATACCCCAATCACCCCTTGTTCGAGCTTAAttactcggtccatctgtaattaagatcaggaaggttagtgtatgtaattta 1664 H S P I P Q S P L V R A 492 gaccaaattgatgcattctttcctgtggtaatagccaataaaacatggaccatttgatcaaaaaaaaaaaaaaaaaaaaaaaaaaaaa 1752 图 1 芍药 ACS 基因的核苷酸序列及其编码的氨基酸序列 下载的论文 Molecular cloning and expression analysis of an 1 aminocyclopropane 1 carboxylate synthase gene from Oncidium Gower Ramsey 为 2016 年发表于 Biochemical and Biophysical Research Communications 的最新英文文章 见下页 2 分 Molecular cloning and expression analysis of an 1 aminocyclopropane 1 carboxylate synthase gene from Oncidium Gower Ramsey Le Song Shi Jin Ping Liu Key Laboratory of Protection and Development Utilization of Tropical Crop Germplasm Resources Hainan University Ministry of Education College of Agronomy Hainan University Haikou Hainan Province 570228 China a r t i c l e i n f o Article history Received 17 November 2015 Accepted 23 November 2015 Available online 26 November 2015 Keywords ACC synthase Ethylene biosynthesis Flower senescence Oncidium Gower Ramsey Gene cloning Expression analysis a b s t r a c t 1 aminocyclopropane 1 carboxylic acid ACC synthase ACS is a rate limiting enzyme in the biosyn thesis of ethylene which regulates many aspects of the plant development and responses to biotic and abiotic stresses In this study a full length cDNA of ACC synthase OnACS2 was cloned from the senescing fl ower of Oncidium Gower Ramsey by RACE The full length cDNA of OnACS2 GenBank accession no JQ822087 was 1557 bp in length with an open reading frame ORF of 1308 bp encoding for a protein of 435 amino acid residues The predicted OnACS2 protein had a molecular mass of 49 1 kDa with pI value of 7 51 Phylogenetic analysis indicated its evolutionary relationships with corresponding orthologous sequences in orchids Hosta ventricosa and monocots Real time PCR assay demonstrated that OnACS2 was constitutively expressed in all tested organs with the highest transcript level in