聚二甲基硅氧烷论文：聚二甲基硅氧烷表面的树状化合物修饰及抗生物粘附分析【中文摘要】聚二甲基硅氧烷(PDMS)由于其廉价、无毒、容易构造、光学透明、气体通透性等优良的化学性质被广泛应用。但是,由于表面强的疏水作用、生物粘附等特性,大大限制了 PDMS 在微流控芯片、生物材料、生物分析中的进一步应用。因此,对 PDMS 表面进行修饰和改性具有重要意义。本文研究了用树状化合物 2-甲基丙烯酸乙氧基苄酯通过原子转移自由基聚合对聚二甲基硅氧烷(PDMS)表面进行修饰的方法。PDMS 表面首先在 H2SO4/H2O2 溶液中氧化,将表面的 Si-CH3 转换成 Si-OH。然后,引发剂通过硅烷化方法固定在表面上,接着树状化合物通过原子转移自由基聚合接枝到 PDMS 表面。修饰后表面的组成和化学状态通过反射红外光谱(ATR-IR)、X 射线光电子能谱(XPS)、接触角表征,表征结果证实树状化合物已成功接枝到 PDMS 表面。进一步,我们通过接触角测定进行树状化合物修饰后表面的稳定性研究;通过 Alexa 594 标记的牛血清蛋白、Alexa 594 标记的卵清白蛋白、FITC 标记的溶菌酶进行修饰后表面的蛋白吸附研究;通过大肠杆菌(E.coli)、金黄色葡萄球菌(S. aureus)进行修饰后表面的细胞粘附研究;通过小鼠胚胎成纤维细胞细胞(NIH/3T3)、海拉细胞(HeLa)进行修饰后表面的细胞粘附研究。研究结果显示,与没有修饰的 PDMS 表面相比,树状化合物修饰后的表面具有很好的湿润性、稳定性,修饰后的表面能在空气中稳定保存 1 个月,同 时修饰后的表面具有显著的抗蛋白吸附、抗细菌粘附和细胞粘附。树状化合物修饰 PDMS 表面后,使表面的性能大幅度提高,这些都是由于树状化合物的结构特征:高灵活度的脂肪族醚、表面亲水性基团、树状结构。修饰后表面良好的性能表明,用树状化合物修饰后的表面可以在生物医学领域中作为抗粘附屏障、抗粘附层、功能化表面被应用。【英文摘要】Recently, PDMS-based application systems have been gaining popularity due to their distinct advantages, such as inexpensive, nontoxicity, easy fabrication, practical scalability, optical transparency, and gas permeability, impermeable to water. However, due to the inherent hydrophobicity of PDMS materials, biological samples easily interact strongly with the PDMS surface when it is present in a biological environment. Moreover, it always prevents the immediate use of PDMS-based microfluidic or bio-and molecular material without any surface processing. Therefore, various surface modification methods have been performed to change the hydrophobicity and biomolecule-adhesion of PDMS surfaces.In this paper, the synthesis of dendronized Poly(3,4,5-tris(2-(2-(2-hydroxyl ethoxy)ethoxy)ethoxy)benzy lmethacrylate) (PEG methacrylate) brushes using surface initiated atom transfer radical polymerization (ATRP) on PDMS substrate was reported. In this approach, the PDMS substrates were first oxidized in H2SO4/H2O2 solution to transform the Si-CH3 groups into Si-OH groups on their surfaces. Then an initiator for ATRP was immobilized onto the PDMS surface, and PG was grafted onto the surface of PDMS via copper-mediated ATRP. Various characterization techniques, including contact angle measurements (CA), attenuated total reflection infrared spectroscopy (ATR-IR), X-ray photoelectron spectroscopy (XPS) ascertained the successful grafting of PG brush at the PDMS surface. Furthermore, the wetability and stability of the PDMS-PolyPG surface were examined by contact angle measurements. The protein adsorption was examined by protein adsorption studies using three fluorophore-labeled protein (Alexa 594-labeled BSA, Alexa 594-labeled Chicken egg albumin, FITC labeled Lysozyme). The ability of these PG brushes to control bacteria adhesion by bacteria adhesion studies using two bacterial species (Gram-positive S. aureus and Gram-negative E.coli). The cell adhesion study of fibroblast cells (NIH/3T3) was used to test the cell adhesion of the PDMS-PolyPG surface. Studies suggest that the PDMS-PolyPG surface exhibited the durable wetability, stability and significantly resist nonspecific protein adsorption, bacteria adhesion and cell adhesion as compared to unmodified PDMS surface. This activity results from its main structural features: a highly flexible aliphatic polyether, hydrophilic surface groups, and branched architecture. The results show that the PDMS-PolyPG surface is probably applicable as an adhesion barrier, antifouling or functional surface in biomedical.【关键词】聚二甲基硅氧烷 树状化合物 表面修饰 原子转移自由基聚合 抗粘附特性【英文关键词】poly(dimethylsiloxane) dendronized compound surface modification SI-ATRP anti-adhesion property【目录】聚二甲基硅氧烷表面的树状化合物修饰及抗生物粘附分析 摘要 6-7 ABSTRACT 7-8 文献综述 11-27 第一章 聚二甲基硅氧烷(PDMS) 微流控芯片的研究进展 11-27 1.1 序言 11 1.2 PDMS 的性质 11-12 1.3 PDMS 表面修饰方法 12-20 1.3.1 气相法 12-14 1.3.2 湿法化学 14-19 1.3.3 气相法和湿化学法相结合的方法 19-20 1.4 PDMS 微流控芯片的应用 20-26 1.4.1 分离生物分子的应用 20-22 1.4.2 固定生物分子的应用 22-24 1.4.3 动物细胞培养中的应用 24-26 1.4.4 在高通量药物筛选中的应用 26 1.5 结论和展望 26-27 本研究的目的和意义 27-28 实验研究 28-50 第二章 树状化合物及其表面引发剂的合成 28-34 2.1 前言 28 2.2 材料 28-29 2.2.1 主要试剂 28-29 2.2.2 主要仪器 29 2.2.3 合成路线 29 2.3 方法 29-31 2.3.1 合成树状化合物 29-31 2.3.2 合成表面引发剂 31 2.4 结果与讨论 31-33 2.5 本章小结 33-34 第三章 PDMS 表面的树状化合物修饰与表征 34-41 3.1 前言 34-35 3.2 材料 35 3.2.1 主要试剂 35 3.2.2 主要仪器 35 3.3 方法 35-36 3.3.1 PDMS 表面修饰 35-36 3.3.