nullnull生物技术通报BIOTECHNOLOGYnull BULLETIN 2009M12B!)sk武善军 荚荣 甘露(v3S833/Li,g230039)null nullKnull1: null 从自然界中分离到一株产木质素降解酶真菌b平板显色反应显示,该菌株具有产多种木质素降解酶的能力,并通过酶活力测定得到证实b为确定该菌株的分类地位,对其 ITS序列进行了扩增并测序,并利用MEGA4. 0生物学软件计算其与同属其它菌株的遗传距离并构建系统发育树,在分子水平上确定它们之间的亲缘关系b试验结果表明,该菌株的 ITS序列与 Irpex lacteus乳白耙菌序列相似度达 99%,且与 Irpex lacteus XSDnull2亲缘关系最近b1oM: null 平板显色null ITS序列null 系统发育树null 乳白耙菌l: 2009null09null03Te:+(1982null),3,V3,Z_:3; Enullmai:l wushan2002 gmailnullcomYT:G,V3=,q; Enullmai:l jiarong ah163null comIsolationand IdentificationaFungusEnable toSecretLigninnulldegradingEnzymesWu Shanjunnull JiaRongnull Gan Lu(School of Lif e Science, Anhui University, Anhui Key Laboratory of Econullengineering and Biotechnology, H efei 230039)null null Abstrac:t null A ligninnulldegrading fungus was isolated in thenaturalbyusnull Thestrain produced awide variety of ligninnulldegrading ennullzymes through the platenullcolor reactionnull wealso assayed its ligninnulldegrading enzymes in liquid culturenull Todetermine the taxonom ic stanulltus of this strain, its ITS sequencewas amplified through PCR reactionnull The genetic distance of severa strains of the same genuswascalculated and a phylogenetic treewas constructed based on ITS sequence by softwareMEGA ( version 4null0)nullThe results demonstratedthat the ITS sequence of this strain had a similarity as high as 99% compared to Irpex lacteus and its closest strain in geneticwas Irpexlacteu s XSDnull2null null Abstrac:t null Platenullcolor reaction ITS sequencenull Phylogenetic treenull Irpex lacteusnull nulljTTBV3,/a?aZ1o,SjTs(missing),|Hydnumrepandum (AJ783968)Sd?( outgroup),i1 000Q1_b2nullTs2. 1 菌株分离与纯化YVs!,V0L8s12),iVQB,B!b2. 2 null 平板显色|sB)c7!p,V|A,Sv)W 1(m1)bB!P7!pA,k:FnullnullMp30 min,d1,A,)?3(Lac);Fp2a3dVAAA,U) 50% )bKimura twonullparameterL.9TV(V1):WL.W1Irpex sp. HZnull13(EU301643)aInull lacteus XSDnull2(EU273517)Inull lactenullus T60(FJ462768)L.K,0null002,Irnullpex spnullF20081106KL.K,90null023b7d(m5)V, W1Inulllacteus95%B,s2, Inulllacteusdd08106(FJ810187)1,6BW1Inulllacteus XSDnull2(EU273517)1KbV1nullk)L.1 2 3 4 5 6 7 8 912 0null0023 0null013 0null0154 0null013 0null015 0null0005 0null021 0null023 0null019 0null0196 0null019 0null021 0null017 0null017 0null0027 0null019 0null021 0null017 0null017 0null002 0null0008 0null019 0null021 0null017 0null017 0null002 0null000 0null0009 0null021 0null023 0null019 0null019 0null004 0null002 0null002 0null00210 0null412 0null408 0null408 0null408 0null411 0null415 0null415 0null415 0null419null null 1. Irp ex spnullSUN mnull99326null16 (FJ750850); 2. Irpex spnullF20081106K (FJ750851); 3. Inull hydnoides SFC 971011null12 (AF479668);4. Inull hydnoides SFC 97125null19 (AF479667); 5. Inull lacteus dd08106 (FJ810187); 6. Irpex spnullHZnull13 (EU301643) ; 7. Inull lacteusXSDnull2 (EU273517) ; 8. Inull lacteus T60 ( FJ462768) ; 9. Inull lacteusW1 10:H ydnum repandum (AJ783968)m5null rDNAnullITSd?(AUbootstrapnull 50%)3null)N ishida18,?7!pU47!p!3M3!?bkPAQ7!pT_!1U4,TAOZEe,VTz!)ZEbV,)W 1s1MnPLiP,Os175生物技术通报Biotechnologynull Bulletin 2009M125 8 db,yN,)Hqb)8cWu( rDNAnullITS)s,VVMQ3T1sf,+saTeL+,y7)s#k19bkITSsZE,?CW1)ITSIrpexlacteus)Mr99%,V7|W1BIrpex lacteusBbyd?V,W 1InulllacteusXSDnull2T1KbID1null,!,#,mnullS3,2008, 28( 2): 66 70null2null,f,nullV, 2005, 5(4): 414 418null3null Christopher HVnull International Biodeterioration & Biodegradnullation,2003, 51: 67 75.4null Lekounougou S, M ounguenguiS, DumarcayS, et alnull InternationalB iodeterioration& Biodegradation, 2008, 61: 287 293null5null MarioCNS, PaulinaM, ConstanzaSL, etalnull ProcessB iochemistry,2008, 43: 368 375null6null Revankar MS, Lele SSnull Process Biochemistry