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424 12Seipp MT,Pattison D,Durtschi JD,et Quadru plex genotyping of F5,F2,and MTHFR variants in a single closed tube by high-resolution amplicon meltingJClin Chem,2008,54(1):108115 13】Vossen RH,Aten E,Roos A,et a1Highresolution melting analysis(HRMA):more than just sequence variant screeningJHum Mutat,2009,30(6):860 866 14Singer-Sam J,Grant M,LeBon JM,et a1Use of a Hpa IIpolymerase chain reaction assay to study DNA methy lation in the Pgk-1 CpG island of mouse embryos at the time of Xchromosome inactivationJ Mol Cell Bio1,1990,10(9):4987-4989 15】Melnikov AA,Gartenhaus RB,Levenson AS,et 01 MSRE-PCR for analysis of gene-specific DNA methyla- tionJNucleic Acids Res,2005,33(10):e93 16Strathdee G,Sim A,Brown RControl of gene ex pression by CpG island methylation in normal cellsJ Journal of Forensic Medicine,December 2013,Vo129,No6 Biochem Soc Trans,2004,32(Pt 6):913-915 17De Smet C,Lurquin C,Leth6 B,et a1DNA methylation is the primary silencing mechanism for a set of germ line-and tumor-specific genes with a CpGrich promoterJMol Cell Biol,1999,19(11): 7327 7335 18Strathdee G,Davies BR,Vass JK,et Cell type- specific methylation of an intronic CpG island con trois expression of the MCJ geneJCarcinogenesis, 2004,25(5):693-701 19Oshiro MM,Futscher BW,Lisberg A,et a1Epige netic regulation of the cell type-specific gene 14-3- 3sigmaJNeoplasia,2005,7(9):799808 20】Juskowiak BNucleic acidbased fluorescent probes and their analytical potentialJAnal Bioanal Chem, 2011,399(9):31573176 (收稿日期:20l20426) (本文编辑:柳燕) 串联质谱法 气相色谱一质谱法气液体色谱法一质量光谱测定法色 谱法,气液质量光谱测定法色谱法,气质量光谱测定 法质量光谱测定法一气相色谱法 气相色谱法 色谱法,高速液相色谱法,高效液相色谱法,高压液相 色谱法液an液相色谱法 间质性肺疾病肺炎,间质性肺炎,间质 肺疾病阻塞性 慢性阻塞性肺疾病慢性阻塞肺疾病 创伤性膈疝 膈疝 腹内疝 动脉瘤样骨囊肿 胃癌 骨髓移植骨髓细胞移植移植,骨髓移植,骨髓细胞移 植术,骨髓 串联质谱法tandem mass spectrometry 气相色谱一质谱法 gas chromatographymass spectrometry 色谱法,气相 chromatography,gas 色谱法高压液相 chromatographyhigh pressure liquid 色谱法液相 chromatography1iquid 肺疾病,间质性lung diseases,interstitial 肺疾病阻塞性lung diseasesobstructive 肺疾病,慢性阻塞性pulmonary diseasechronic obstructive 疝,横膈,创伤性hernia,diaphragmatic,traumatic 疝,横膈hernia,diaphragmatic 疝。腹部herniaabdominal 骨囊肿,动脉瘤样 bone cystsaneurysma1 胃肿瘤 stomach neoplasms 骨髓移植 bone marrow transplantation
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