Dataset S2. Molecular analysis of the medfly sperm-marked strain 1260_F-3_m-1 for verifying transgene stabilization1) Original genomic situation at the integration site of the 5 pBac endOriginal strain 1260_F-3_m-1: primer pair 380/229ggcccacatttgtctaacttctgttacgtggttcagtagtgagttttgagtaatagcctaaatgtagatgtttaaCCCTAGAAAGATAGTCTGCGTAAAATTGACGCATGCATTCTTGGAATATTGCTCTCTCTTTCTAAATAGCGCGAATCCGTCGCTGTGCATTTAGGACATCTCAGTCGCCGCTTGGAGCTCCCGTGAGGCGTGCTTGTCAATGCGGTAAGTGTCACTG2) Verification of the site-specific integration in 1260_F-3_m-1attR-site of the integrated strain int_1260_F-3_m-1: primer pair 373/372ATCTTGACCTTGCCACAGAGGACTATTAGAGGTAAGAATAAACATTGTTGGTCAACTTCAAAGTCCACGAGGCGTAGCCGAGTCTCTGCACTGAACATTGTCAGATCTGGCTAAATGGTATGGCAAGAAAAGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAACGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAACGGGTATGCAATATAATAATCTTTTATTGGGTATGCAACGAAAATTTGTTTCGTCAAAGTATGCAATATTTTTTATTAAAAGAGGGTATGCAATGTATTTTATTAAAAACGGGTATGCAATAAAAAATTATTTGGTTTCTCTAAAAAGTATGCAGCACTTATTTTTTGATAAGGTATGCAACAAAATTTTACTTTGCCGAAAATATGCAATGTTTTTGCGAATAGGGTACCGGCGCGCCTAGGCCGGCCGAATTCGTGCCCCAACTGGGGTAACCTTTGGGCTCCCCGGGCGCGTACTCCACCTCACAGATCTCGAGCTCAAattL-site of the integrated strain int_1260_F-3_m-1: primer pair 330/382GCTCATCGACTTGATATTGTCCGACACATTTTCGTCGATTTGCGTTTTGATCAAAGACTTGAGCAGAGACACGTTAATCAACTGTTCAAATTGATCCATATTAACGATGGGCCCGGTACCCTATTCGCAAAAACATTGCATATTTTCGGCAAAGTAAAATTTTGTTGCATACCTTATCAAAAAATAAGTGCTGCATACTTTTTAGAGAAACCAAATAACTTTTTATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACTTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACGTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCTTTTCTTGCCATACCATTTAGCCGGATCCTGCGGGTGCCAGGGCGTGCCCTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCAAT3) Verification of remobilization and loss of piggyBac ends Stabilized strain stab_1260_F-3_m-1: primer pair 380/382ggcccacatttgtctaacttctgttacgtggttcagtagtgagttttgagtaatagcctaaatgtagatgtttaaATAATAGTTTCTAATTTTTTTATTATTCAGCCTGCTGTCGTGAATACCGTATATCTCAACGCTGTCTGTGAGATTGTCGTATTCTAGCCTTTTTAGTTTTTCGCTCATCGACTTGATATTGTCCGACACATTTTCGTCGATTTGCGTTTTGATCAAAGACTTGAGCAGAGACACGTTAATCAACTGTTCAAATTGATCCATATTAACGATGGGCCCGGTACCCTATTCGCAAAAACATTGCATATTTTCGGCAAAGTAAAATTTTGTTGCATACCTTATCAAAAAATAAGTGCTGCATACTTTTTAGAGAAACCAAATAATTTTTTATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACTTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCCGTTTTTAATAAAATACATTGCATACCCTCTTTTAATAAAAAATATTGCATACGTTGACGAAACAAATTTTCGTTGCATACCCAATAAAAGATTATTATATTGCATACCTTTTCTTGCCATACCATTTAGCCGGATCCTGCGGGTGCCAGGGCGTGCCCTTGAGTTCTCTCAGTTGGGGGCGTAGGGTCAAT