? ? ? ?!? “#$?%?&?“?%?&? ?!“? ?!“ ?#?$ #$%?#?$ #$% ? ?%?&?“?$ “?“?$ “? ? ?“$% ?%?“$% ?%? ?&? ?!$ ?“%?!$ ?“%? ()*?)?*?&?“ ?“%“?#$ ?“%? ?$“? ?!?#?$“# ?!?“ ?&?%? ?%#?%?$ ?%#? +?,?&?“ ?!?#? -?$? ?#? ?“? .?&? ? ?#?“ ?/(?&?#%?“ #$“?“ #?0?*?,? ? ? ? ? ?pDONR221 4761 bp Restriction/Methylation Map Enzyme # of cuts Positions AccI 4804 852 1754 2749 AccIII 12292 AciI 2743 64 71 114(c) 138 148 204(c) 238 324(c) 337 375 384(c) 1246(c) 1585 1780 1841(c) 1948(c) 2312(c) 3186 4164 4173(c) 4308 4418(c) 4539(c) 4558(c) 4685(c) 4713(c) AcsI 32288 3194 3378 AcyI 1328 AflII 1555 AflIII 3231 4060 4756 AluI 1957 175 560 663 1324 1687 2263 2392 2511 2520 2892 2994 3033 3047 4030 4199 4456 4502 4592 Alw44I 21386 4442 AlwI 81409 1828(c) 1841 3443(c) 3838 4110(c) 4112 4198 AlwNI 21514 4347 ApaI 1568 ApaLI 21386 4442 ApoI 32288 3194 3378 AseI 3167 226 3780 AsnI 3167 226 3780 Asp700 11053 AspEI 3550 800 2756 AspHI 21390 4446 AvaI 2561 1417 BamHI 11833 BanII 2568 3236 BbsI 1438(c) BbvI 1185 103 184 826(c) 1703(c) 2723(c) 3056 4128(c) 4334(c) 4337(c) 4427 BfaI 4241 507 1015 4263 BfrI 1555 BmyI 4568 1390 3236 4446 BpmI 12172(c) BpuAI 1438(c) BsaAI 12599 BsaBI 11406 BsaHI 1328 BsaI 11277(c) BsaJI 101395 1417 1987 2056 2057 2635 2636 3452 3854 4596 BsaWI 51646 2292 3716 4403 4550 BseAI 12292 BsiEI 585 808 856 3581 4422 BsiHKAI 21390 4446 BsiYI 14154 346 1279 1417 1598 2063 2630 3495 3827 3844 4278 4557 4723 4741 BslI 14154 346 1279 1417 1598 2063 2630 3495 3827 3844 4278 4557 4723 4741 BsmAI 61277(c) 1358(c) 2064(c) 2617 3064 3597 BsmFI 22672 4032 BsmI 41894(c) 2301(c) 3465(c) 3542(c) Bsp120I 1564 Bsp1286I 4568 1390 3236 4446 BspEI 12292 BspHI 23105 4032 BspMI 11171 BspWI 1463 130 214 1563 1858 1954 2517 2721 3050 3293 3325 3539 4138 4710 BsrBI 266(c) 377(c) BsrDI 4633 2275(c) 2924(c) 3079(c) BsrFI 21286 3535 BsrGI 3652 1442 2899 BsrI 16201 442(c) 524(c) 550(c) 758(c) 1175 1299 1395 2047 2487(c) 2799 3372 3996 4229 4341(c) 4354(c) BssHII 11792 Bst1107I 11755 BstNI 14246 311 479 1396 2058 2114 2637 2665 3041 3471 3828 4597 4610 4731 BstUI 13150 152 233 498 1246 1794 1841 3188 3238 3583 4062 4132 4713 BstXI 11306 BstYI 41833 3830 4104 4115 CfoI 1891 124 152 217 1320 1794 1796 2717 3296 3518 3535 3605 4026 4132 4241 4415 4515 4582 Cfr10I 21286 3535 Csp6I 7235 653 1443 1874 2412 2900 3415 DdeI 14298 398 454 779 847 1253 1562 2068 2516 2744 2773 3598 4073 4482 DpnI 131258 1403 1835 2563 2606 2690 3450 3580 3832 4098 4106 4117 4192 DpnII 131256 1401 1833 2561 2604 2688 3448 3578 3830 4096 4104 4115 4190 DraI 22033 2372 DraII 1565 DrdI 14654 DsaI 11987 DsaV 24244 309 346 379 477 1346 1394 1416 1417 1552 2056 2112 2552 2635 2644 2663 3039 3452 3469 3826 4377 4595 4608 4729 EaeI 5156 547 1296 1392 2023 Eam1105I 3550 800 2756 EarI 3112(c) 244(c) 3394 Eco57I 14214(c) EcoNI 13493 EcoO109I 1565 EcoRI 12288 EcoRII 14244 309 477 1394 2056 2112 2635 2663 3039 3469 3826 4595 4608 4729 EcoRV 12999 Esp3I 32064(c) 2617 3597 Fnu4HI 1771 74 92 173 840 1717 1842 1949 2737 3045 3186 4142 4348 4351 4416 4559 4714 FnuDII 13150 152 233 498 1246 1794 1841 3188 3238 3583 4062 4132 4713 FokI 11262(c) 298 527 1315(c) 1394(c) 1432(c) 1554(c) 2308 2974(c) 3219 3825(c) HaeII 2125 4516 HaeIII 21158 274 290 351 440 549 566 1298 1394 2025 2112 2334 2379 2634 3053 3185 3668 4282 4716 4734 4745 HgaI 5317(c) 2713(c) 4051(c) 4066(c) 4644(c) HgiAI 21390 4446 HhaI 1891 124 152 217 1320 1794 1796 2717 3296 3518 3535 3605 4026 4132 4241 4415 4515 4582 Hin