Copyright2009-2012GENORISE SCIENTIFIC, INC.ordergenorise.comCatalog 101074Cat.NO.Cat.NO.Cat.NO.Cat.NO. 101074101074101074101074 Store the kit at 15 to 25GenoriseGenoriseGenoriseGenorise WholeWholeWholeWholeBloodBloodBloodBloodDNADNADNADNA15min15min15min15min KitKitKitKit 50505050ForForForFor DNADNADNADNApurificationpurificationpurificationpurificationfromfromfromfrom wholewholewholewhole bloodbloodbloodbloodFor Research Use onlyCopyright2009-2012GENORISE SCIENTIFIC, INC.ordergenorise.comCatalog 101074WholeWholeWholeWholeBloodBloodBloodBlood GeGeGeGen n n nomicomicomicomicDNADNADNADNA PurificationPurificationPurificationPurification1 1 1 1P P P PreparereparereparerepareBufferBufferBufferBuffer GLGLGLGLAddAddAddAdd500500500500 L L L L BufferBufferBufferBufferGLGLGLGL2 2 2 2LyseLyseLyseLyse samplesamplesamplesampleAddAddAddAdd200-250200-250200-250200-250 L L L L wholewholewholewholebloodbloodbloodbloodVortexVortexVortexVortex30303030 SecSecSecSec3 3 3 3proteinproteinproteinprotein precipitationprecipitationprecipitationprecipitationAddAddAddAdd100100100100 L L L L BufferBufferBufferBufferGPPGPPGPPGPPVortexVortexVortexVortex30303030 SecSecSecSec4 4 4 4proteinproteinproteinprotein precipitationprecipitationprecipitationprecipitation12,00012,00012,00012,000g g g g5 5 5 5minminminmin5 5 5 5BindBindBindBind DNADNADNADNA6 6 6 6WashWashWashWash SilicaSilicaSilicaSilica MembraneMembraneMembraneMembrane7 7 7 7WashWashWashWash SilicaSilicaSilicaSilica MembraneMembraneMembraneMembrane8 8 8 8WashWashWashWash SilicaSilicaSilicaSilica MembraneMembraneMembraneMembrane12,00012,00012,00012,000g g g g1 1 1 1minminminmin1 1 1 1WashWashWashWash500500500500 L L L LGW1GW1GW1GW12 2 2 2WashWashWashWash500500500500 L L L LGW2GW2GW2GW2 ; ; ; ;700700700700 L L L LGW2GW2GW2GW212,00012,00012,00012,000g g g g1 1 1 1minminminmin9 9 9 9DryDryDryDry SilicaSilicaSilicaSilicaMembraneMembraneMembraneMembrane12,00012,00012,00012,000g g g g2 2 2 2 minminminmin10101010EluteEluteEluteEluteHighlyHighlyHighlyHighlyPurePurePurePureDNADNADNADNA50505050 L L L L BufferBufferBufferBuffer GE(70GE(70GE(70GE(70) ) ) )RTRTRTRT1 1 1 1 minminminmin12,00012,00012,00012,000g g g g1 1 1 1 minminminminKitKitKitKitContentsContentsContentsContents GenoriseGenoriseGenoriseGenorise WholeWholeWholeWhole BloodBloodBloodBlood DNADNADNADNA miniminiminimini KitKitKitKit50 Cat No.101074DNA binding columns50 Collection Tubes50 Buffer GL30ml Buffer GPP6mlBuffer GW119ml +12ml ethanolBuffer GW219ml +46ml ethanolBuffer GE12mlCopyright2009-2012GENORISE SCIENTIFIC, INC.ordergenorise.comCatalog 101074 2 2 2 2Copyright2009-2012GENORISE SCIENTIFIC, INC.ordergenorise.comCatalog1010747 7 7 76. 6. 6. 6.TroubleshootingTroubleshootingTroubleshootingTroubleshooting-NONONONOororororpoorpoorpoorpoorDNADNADNADNAyieldyieldyieldyieldIncompleteIncompleteIncompleteIncompletelysislysislysislysis Low WBC content in blood sample Inefficient lysis with Buffer GL Inefficient mixing with Buffer GPP DNA not efficiently eluted Blood coagulation EthanolEthanolEthanolEthanolnotnotnotnotaddedaddedaddedaddedto to to to GW1andGW1andGW1andGW1and BufferBufferBufferBuffer GW2GW2GW2GW2 Add absolute ethanol to the buffers before using. After adding ethanol, mix the buffers well and store at +15 to +25. Always mark GW1vial and Buffer GW2 vial to indicate whether ethanol has been added or not. -PurifiedPurifiedPurifiedPurified DNADNADNADNAsamplesamplesamplesample cannotcannotcannotcannot easilyeasilyeasilyeasily bebebebe loadedloadedloadedloaded intointointointo thethethethe wellwellwellwell of of of of anananan agaroseagaroseagaroseagarose gel,gel,gel,gel, butbutbutbutinsteadinsteadinsteadinstead “pops“pops“pops“pops out“out“out“out“ of of of ofthethethethe wellwellwellwell asasasas it it it it is is is is loaded.loaded.loaded.loaded.EluateEluateEluateEluate containingcontainingcontainingcontaining thethethethe purifiedpurifiedpurifiedpurified DNADNADNADNAproductproductproductproductis is is is contaminatedcontaminatedcontaminatedcontaminated withwithwithwith ethanolethanolethanolethanol fromfromfromfrom thethethethe WashWashWashWash Buffer.Buffer.Buffer.Buffer.After the last wash step, make certain flowthrough solution containing Wash Buffer does not contact the bottom of the Filter Tube. If this has occurred, empty the Collection Tube and reinsert the contaminated filter, and re-centrifuge for 30 seconds. -