Using This Template (Research Article general)The template consists of essential headings along with body text explaining what to include in each section. You should overwrite (or copy and paste) the body text with the corresponding section text for your article. Obviously, you should add other headings as needed.Title (150 characters or fewer)The title should be specific to the study yet concise, and should allow sensitive and specific electronic retrieval of the article. It should be comprehensible to readers outside your field. Avoid specialist abbreviations if possible. Present this in title case, capitalizing all words except for prepositions, articles, and conjunctions. Titles should also include relevant information about the design of the study, e.g.: Television watching and family dysfunction in medical journal editors: a case-control study. Authors and AffiliationsProvide the first names or initials (if used), middle names or initials (if used), surnames, and affiliationsdepartment, university or organization, city, state/province (if applicable), and countryfor all authors. One of the authors should be designated as the corresponding author. If the article has been submitted on behalf of a consortium, all author names and affiliations should be listed at the end of the article.AbstractThe abstract succinctly introduces the paper and outlines the fundamental task the software accomplishes, examples of biological insights from the use of the software and details of availability, including any operating system dependencies. We advise that it should not exceed 250300 words. Please do not include any citations in the abstract. Avoid specialist abbreviations if possible. IntroductionThe introduction should put the focus of the manuscript into a broader context, describing the problem addressed by the software. As you compose the introduction, think of readers who are not experts in this field. Include a brief review of the key literature, establishing or optimizing algorithms used in the software and/or to papers demonstrating their relevance to the biological problem addressed by the software. The introduction should conclude with a brief statement of the overall aim of the experiments and a comment about whether that aim was achieved. ResultsThe results section should provide details of all of the experiments that are required to support the conclusions of the paper. Authors should include examples of biological problems solved using the software, including results obtained with the deposited test data and associated parameters. There is no specific word limit for this section. The section may be divided into subsections, each with a concise subheading. Large datasets, including raw data, should be submitted as supporting information files; these are published online alongside the accepted article. We advise that the results section be written in past tense. Design and ImplementationThis section should provide details of the algorithms used by the software and describe how those algorithms have been instantiated, including any dependencies. Authors should also include details of the supplied test data and explain how to install and run the software. A brief description of enhancements made in the major releases of the software may also be given here.Availability and Future Directions Authors should provide details in this section of where the software has been deposited, including the relevant URLs. This section should also detail any future work that the authors plan to carry out and suggest ways in which others might extend the software.AcknowledgmentsPeople who contributed to the work but do not fit criteria for authorship should be listed in the Acknowledgments, along with their contributions. It is the authors responsibility to ensure that anyone named in the acknowledgments agrees to being so named. Details of the funding sources that have supported the work should be confined to the funding declaration provided on submission. Do not include them in the acknowledgments.ReferencesOnly published or accepted manuscripts should be included in the reference list. Meetings abstracts, conference talks, or papers that have been submitted but not yet accepted should not be cited. Limited citation of unpublished work should be included in the body of the text only. All personal communications should be supported by a letter from the relevant authors. PLoS uses the numbered citation (citation-sequence) method. References are listed and numbered in the order that they appear in the text. In the text, citations should be indicated by the reference number in brackets. Multiple citations within a single set of brackets should be separated by commas. Where there are more than three sequential citations, they should be given as a range. Example: “. has been shown previously 1,4-6,22.” Make sure the parts of the manuscript are in the correct order before ordering the citations.Because all r