ABI Support TrainingABI Support Training|Shimadzu Scientific InstrumentsprominenceLiquid ChromatographProminence Set-up and ConfigurationFeeding the MSprominenceLiquid ChromatographABI Shimadzu Relationship|Sales Agreement|ABI can sell Shimadzu HPLC with ABI MS|SSI sales fully compensated|Demo Lab Support|Toronto Demo lab projects with Dr. Sakuma|Foster City and Framingham support|PresentationsControl of the Prominence HPLCThere are 3 ways to interface and control a Shimadzu HPLC CBM-20A CBM-20A Lite SIL-HT (SCL-10Avp) Communications settings are the same for all three sort ofControl of the Prominence HPLC Please communicate with your local Shimadzu reps to coordinate installations and training. The Shimadzu Engineer will have either already set-up the HPLC or, ideally, is there with you to assist in the installation. Shimadzu is your partner in this endeavor. Please use the resources available to you. Having said this, we all no that the world is not an ideal place and “stuff happens”Setting Comms SIL-HT(SCL-10Avp)From MENU Screen: Press 4 (system) Set RELAY 1 to start with arrow keys Press F3 (next) Set: CLASSvp to 5 or 6 Interface to RS-232C Baud Rate to 19200 Level to ENHANCED POWER CYCLE the unit to save settings ROM version 5.33 OR LATER is acceptable.Setting Comms CBM-20A (Lite) From the front panel of either a pump or autosampler where the CBM Lite is installed: Press VP key 4X to CALIBRATION Press FUNC Enter 00000 (five zeroes) followed by ENTER Press BACK Press ENTERSetting Comms CBM-20A (Lite) Press FUNC 2X and begin entering the following. After entering a value press enter, then FUNC to move to next parameter. INTERFACE: 1 for RS-232C 2 for Ethernet (PREFERRED) ETHERNET SPEED 0 for auto-detectSetting Comms CBM-20A (Lite) The following 4 settings should be set up by the Shimadzu engineer USE GATEWAY (only if connected to network) 0 for NO 1 for YES IP ADDRESS SUBNET MASK DEFAULT GATEWAY Important to you: TRS MODE 2 for ANALYST communication POWER CYCLE the unitSupplied by customers IT groupPreferred to use Ethernet for Comms We have recently learned that the MIMIC Driver in Analyst does not work with USB/RS232 converters. If RS232 is desired please use a Serial expansion board Better to add another Network Card and set it up as a peer-to-peer network.Setting IP address on PCRight click My Network Places, “properties” Right click on the desired Local Area Connection Select PROPERTIES Highlight Internet Protocol (TCP/IP) Click PROPERTIES Click on “Use the following IP address” and fill in the infoSettings for Peer-to-PeerDefault settings on CBM-20A (Lite) IP Address: 192.168.200.99 Subnet Mask: 255.255.255.0 Default Gateway: Not UsedSet the PC up as a “different house on the same street” IP Address: 192.168.200.97 Subnet Mask: 255.255.255.0 Default Gateway: Not UsedProminence Patch Ready for release in December of 2005 MUST have previous patches (April and July) installed. Old Methods and Configurations DO NOT work. Methods must be re-created Remind customers to review RELEASE NOTESProminence Patch If Upgrading to Prominence Patch make sure all units in LC-20 mode NOT VP VP CalibrationOP MODE20A Quick Check look for all remote LEDs to be lit to ensure proper link to controller. Make HPLC run longer than MS run - 30 Sec.Analyst 1.4.1 Set-upAnalyst 1.4 on WindowsXP SP2Analyst 1.4 on WindowsXP SP2System Controller SelectionSystem Controller ConfigurationSystem Controller ConfigurationSystem Controller ConfigurationPump SelectionPump Configuration Pump Configuration Detector SelectionDetector Configuration - UVColumn Oven SelectionColumn Oven ConfigurationSub-Controller SelectionSub-Controller ConfigurationAutosampler SelectionAutosampler Configuration SIL-HTAutosampler Configuration SIL-20Ready to RUN Setting up a method in Analyst Some Shimadzu info and tipsLC SyncPump Set-upPump Set-upDetector Set-upAutosampler Set-upAnalyst does not support Control Rack!Oven Set-upFCV-12AH Positions123564123456Position “0”Position “1”EVENT Checked means contact closed at initial conditionsTime ProgramTime ProgramSolvent Selection Set-upWiring Connections Sciex General connection cable 25 pin Green/ Black and White/Black (pins 21 & 22) are connected to any of the Shimadzu relays typically RELAY/EVENT 1 for a start signal. Must Short out (twist the pair together) Pins 9 (Red/Black) & 10 (Orange/Black) standard procedure for Sciex installs.Resources Available to youYour local Shimadzu support structure Sales Tech support Service Regional Office We can provide you with names and numbers 9 Regional offices 50 Sales people 30 Tech Support 40 ServiceThey are all compensated on ABI sales and are available to help you.Shimadzu Corporate 800-477-1227 Extension: 1300 Gets Customer Service to direct the call to Phone Tec