嗜热嗜酸菌Thermophilic Acidophilic Bacilli ：TAB主要内容oo关于关于 TAB TAB （特性特性, , 适宜的生长温度，适宜的生长温度，pHpH值值 . .）ooTABTAB导致的问题：对饮料的影响导致的问题：对饮料的影响 （气味，气味， 氧化氧化etc.etc.）oo检测检测TABTAB方法方法 （怎样检测怎样检测）oo防治防治TABTAB的方法的方法oo武汉厂武汉厂TABTAB检测现状及分析（检测现状及分析（生产低的生产低的pHpH 产品的必要知识和信息产品的必要知识和信息）在饮料行业中关于在饮料行业中关于TAB的研究报告的研究报告o年 Cerny : Isolated TAB from accident products of clear apple juice.o年 Darland : Genericly Named B. Acidoterrestris.o年 Suzuki : Found Rotten canned low pH commodities odoring in the market, reported D95 (Decimal reduction time at 95)= 2min.o年 Niwa : Suggested K mediums to isolate TAB,isolating TAB from imported juices.o年 Kato : Analyzed elements of offensive smells caused by TAB. Reported the process;Vanillin Guaiacol, aldehydealcohol”.o年 Niwa : Developed YSG mediums.o年 Matsubara : Reported the way to detect A. Acidoterrestris with PCR.o年 Niwa : Reported the way to detect TAB with peroxydase.TAB特性o1.菌体(宽 0.71m, 长35m) 嗜酸耐热菌的菌落形态及显微形态TAB特性o2.生长温度范(2070)最适生长温度 : 4060o3.生长pH范围(pH 26)最适 pH : 3.54.5TAB来源contamination from soil （and fruits, fructose）SoilSoilSoilSoilSoilSoft drinksJuiceJuiceHerbMaterialsSoil, Fruits, fructoseSoil, Fruits, fructoseSoil, Fruits, fructoseIn JapanFoundFoundFoundFoundFoundNot FoundNot Found Not FoundNot Found Not FoundNot FoundNot FoundNot FoundIsolated fromFrequencySpeciesRareRareRareRareRareRare RareRareRareRare SeveralManyManyTAB简介oo分类分类: : 需氧革兰氏阳性菌需氧革兰氏阳性菌oo特性特性: : 芽孢杆菌芽孢杆菌oo耐热度耐热度: : minminoo生长温度生长温度 : : 20207070（适宜适宜 : 45: 45 6060）oo生长生长 pH:pH:（适宜适宜r : 3.5r : 3.54.54.5）oo代谢物代谢物: : 多种物质，多种气味多种物质，多种气味oo分布分布：土壤及其他环境土壤及其他环境防治饮料中的TAB的方法o提高灭菌温度 oUHT sterilization: over F0=1o保持原料和商品低温. 现阶段我们（供应商/运输/储存）是如何做的？o在 10以下保存冷冻 （目的是什么？）o控制氧气 我公司产品的含氧量控制？oHigh risk container：PET（most dangerous）, paperoCan Drinks：Control Sealed Oxygen 2ml/350ml.（现在产品含 氧量）o控制每一步条件.oPlan pH of content under 3.2（Effective with Apple or Orange)我们现在原物料PH：oUse Sodium Benzoate.oUse the ex. from seeds of grapes or other poly- phenols.(with dregs)oWe confirm whether odor is with multiplication of TAB.oavailable measures are spread to material suppliers in Japan. TAB的检测方法o嗜热嗜酸菌TAB的检测 .doc生产低的生产低的pHpH产品的必要知识和信息产品的必要知识和信息The points in planning commoditiesWhether would TAB grow up？（Confirm with inoculating PET products）How is the influence of TAB to the commodities？（flavor, appearance）Does the container control O2？（Both PET and Paper arent available.）Can we deal as the chilled commodities？ The points of controlling materialsWhat materials contain TAB？（Juice, Saccharide etc.）The materials have to be produced via or removing TAB processes.Dont reuse Water in a loop line for washing materials.How to inspect materials（ plates, the Peroxidase methods）Every standard of material The points of controlling commoditiesThe commodities are also produced via or removing TAB processes.How to inspect commodities（ plates, and others）生产低的生产低的pHpH产品的必要知识和信息产品的必要知识和信息Get sterilizing temperature up： Preserve Materials and commodities at low temp. Chilling distribution ( under 10) Use Materials with absorption or untransmittance of O2. Unavailable container: ：, PaperCan：Control Top of O2 to Plan commodities considering TAB control with contents. Dropping pH under 3.2（effective to Oranges and Apples） Adding Sodium benzoate. Adding extracts of grape seed or polyphenol powder（sediments） Inoculating TAB and confirm the multiplying in risky commodities. Remove contaminated materials at the step of purchase. Material supplier are opened the skill they can apply.