S/Tobacco Science& Technology/Tobacco Chemistry 2005M4(9213): :0371_67672637, E_mail:llqmail.tobaccoinfo.com.cn.烟草蛋白质研究进展u,S,(华南理工大学食品与生物工程学院,广州市天河区五山街 510640)1oM:烟草;蛋白质;生物活性肽;香精;香料K1:综述了烟草蛋白质的营养价值a提取方法a功能特性(包括烟叶硒蛋白和生物活性肽),以及以烟叶蛋白为原料生产普通的蛋白食品a功能性食品a香精香料a转基因蛋白a医药学研究辅助材料和植物保护剂等,并展望了烟草蛋白的开发利用前景bms|:TS411.1 DSM:A cI|: 1002- 0861(2005)04- 0031- 04Advance in Tobacco ProteinZHAOMOU-MING, RAO GUO-HUA, and LIN WEI-FENGCollegeof Food and Bioengineering, South ChinaUniversity of Technology, Guangzhou 510640, ChinaKeywords: Tobacco; Protein; Bio-active peptides; Essence; FlavorAbstract: Thenutritionalvalue, extraction and functional characteristics (including selenium protein and bio-activepeptides) of tobacco protein and the protein food, functional food, essence and flavor, transgenicprotein, medicalresearch ancillary material and plant protectant made from tobacco protein were reviewed. The prospects of tobaccoprotein development and utilization were discussed also.1 !N=s,cK,cr20. 48% 1, 2b=sVbVBP=s8(Fraction I protein, FI),6BV(Fraction II protein, FII) 3bV$X(Nicotiana tabacum L. cv.NC89)7=?4|VV1U4b-,vi4TL2c,1=7?b=,vM=WK,%-Wgmn5BJ5bBt 3,5,6TV,=Vc,FIAc(WjF(FAO)AcS(V2) ,a&daVS1P,1Bt1JTalaavAc,V=FI!Nb=1ad/a8l,!N0NbV1 7=V4|4 ( g/kg) Y4s4V15.98 13.77 11.39FI7.65 6.48 5. 12FII8.33 7.29 6. 2731V2 =FIBtJTAF4 (g/ 100g) FIFAOSlv?8.25.5 2.8 3.8 3.3 3. 7 3. 93.1!11.25.3d6.26.2 4.2 6.2 4.3 5. 3 5. 21.5 1.4 1.3 1.2 0. 6 1. 23.2 2.2 3.0 2.5 3. 1 1. 1s4.8 4.2 5.2 4.0 6. 4 5. 89.1 4.8 8.2 7.0 15. 0 7. 66.9 2.8 5.7 4.0 6. 0 3. 2&d6.2 2.8 5.0 5.1 5. 0 4. 86.1 4.2 3.2 2.7 2. 3 6. 6F2.98.95.32 4|*T4|FI,O3,gv,4s3b,ST=FI4B 7- 9,BFI,KungTso10aLowe11=FI4BZEeSvDb7,t4|ZEs,OvV(e0 4eP;gaarZE4|FIbtZE?BFI,HW4|,4LCv?=bbHq/,V/,-g/M1,/vl: c; ,Jgb6,)Ar 25,26b4.2 生产功能性食品或香料=M,|=Ve),VB3tbs)s/as)svds0s/sBaiS,MF),VB?J27;|Q=Aa?3MaillardQ,ViTPaTTPaKPaPa0PPJP,tP?P,9FP,hO,P?,i?xP,aFP,V,z.F)t+#- J .S0Y,1998,14(3):209- 211.20 Xu hui, Montoya Femando U, Wang Zeping, et al. Combineduseof regulatoryelementswithin thecDNA toincreasethe produc-tion of a soluble mousesingle-chain antibody, scFv, from tobaccocell suspension culturesJ. Protein Expres. Putific., 2002, 24:384- 394.21 Li Qingshun, Lawrence C B, Davies H M, et al. A tridecapep-tide possesses both antimicrobial and protease-inhibitory activitiesJ . Peptides, 2002,23:1- 6.22 Cary J W, Rajasekaran K, Jaynes JM, et al. Transgenicexpres-sion of a geneencoding a syntheticantimicrobial peptide results ininhibition of fungal growth in vitro and in planta J . Plant Sci.,2000, 154: 171- 181.23 CoventryH S, Dubery IanA. Lipopolysaccharides from Burkholde-ria cepacia contributeto an enhanced defensivecapacityand the in-duction of pathogenesis-related proteins in Nicotianae tabacum J.Physi. Molecular Plant Path. , 2001, 4: 149- 158.24 .3J J .S,2000,6(2):14.25 Guenoune Dana, AmirRachel, Ben-Dor Bruria, et al. A soybeanvegetativestorageprotein accumulates to high levels in various or-gans of transgenictobacco plantsJ. Plant Sci., 1999, 145: 93- 98.26 TakaiwaFumio, Katsu