“)”?Z#1rsc陈玉胜1 ,阮鸣1 ,张李阳1,饶玉鹏1 ,孙明丽2(1.2k3S,2211171;2.2D0v,2210029)K1目的对芝芪菌质的不同发酵配方进行筛选,并且对芝芪菌质中总皂苷和粗多糖含量进行测定,从而为制定质量标准做前期准备。方法芝芪菌质的水提醇沉的浓缩液用正丁醇萃取出总皂苷;滤渣用于测定粗多糖;依照05版药典规定用紫外分光光度法测定芝芪菌质中总皂苷和粗多糖的含量。结果 采用紫外分光光度法测定芝芪菌质中总皂苷和粗多糖的平均含量,分别为0.243 3%和1.1616%。结论含2.5%黄芪药材的芝芪菌质效果更优。1oM总皂苷;粗多糖;含量测定;发酵;芝芪菌质ms|Q946DSMAcI|0517-6611(2008)04-01310-02Studyon theScreeningof FermentationFormulaforZhiqiFungalSubstanceand ItsContentDeterminationofMainEffectiveComponentsCHENYu-shengetal(Department of LifeScience,XiaozhuangCollege, Nanjing, Jiangsu 211171)AbstractObjective Theresearch aimedtoscreendifferent fermentation formulaforZhiqi fungal substanceand determinethecontents of total saponinand crudepolysaccharideinordertomakeprophasepreparationsforestablishingqualitystandard. Method Total saponinwas extractedbyN-butanolfromconcentrated solution of Zhiqi fungal substanceafterwaterextraction and alcohol sedimentation.Filterresiduewas used todeterminethecontent of crudepolysaccharide.Accordingto the regulation in thepharmacopoeia(2005edition), thecontentsof total saponincrude polysaccharide inZhiqi fungal sub-stanceweredetermined by using ultraviolet spectrophotometry . Result The average contents of total saponin and crude polysaccharidein Zhiqi fungalsubstancewere determined by usingultraviolet spectrophotometry, being 0.2433%and 1.161 6%resp. Conclusion The effect of Zhiqi fungal sub-stancecontained 2.5%medicinal materials Huangqi was better.KeywordsTotal saponin;Crudepolysaccharide;Content determination ;Fermentation;Zhiqi fungal substance_?$ 1-2 ,Z3F4),2(Ganoderma lucidum)T?),+0(Astragalus membranaceus)0?T0,j!(),ZB?F,+Hq/_?,?0)。!0)?0)。_?_P;_)34!,3)8#Q3T,y)7M0sT。yN,30)|V?cs0r。T0)90.01%,Ov0)(Basidiomycotina)0)(Ascomycotina)。M,vM0F)、8、9CAm44|QqYFig.4Effectsof extractiontimesonpolysaccharideyieldV24|kL9(34)Table2OrthogonaltestL9(34)forextractionof Portabella polysaccharideI|No.A4|QExtractiontimesB1Material/waterratioC4|HWExtractiontimehD4|ExtractiontemperatureqPolysaccharideyield%1 1 120 1.5 80 2.212 1 130 2.0 90 2.853 1 140 2.5 100 2.774 2 120 2.0 100 2.695 2 130 2.5 80 3.016 2 140 1.5 90 2.817 3 120 2.5 90 2.818 3 130 1.5 100 2.889 3 140 2.0 80 2.80K1 7.83 7.71 7.9 8.02K2 8.51 8.74 8.34 8.47K3 8.49 8.27 8.61 8.34K1 2.61 2.57 2.63 2.67K2 2.84 2.91 2.78 2.82K3 2.83 2.76 2.87 2.78R 0.23 0.34 0.24 0.15D,4|KDFB2C3A2D2 。3YV4E4|ykk,4|KDF:1130,HW2.5h,4|2Q,90 。ID 1 BEELMAN RB, ROYSED,CHIKTHIMMAHN.Bioactive componentsin A-garicus bisporus(J.LGE)imbachof nutritional, medicinal, orbiological im-portance J .J.of MedicinalMushrooms, 2003,5:321-337. 2 常明昌.食用菌栽培学M .北京:中国农业出版社,2003. 3 葛玉,肖红,张欣,等.真菌多糖的研究开发现状 J .食品研究与开发,2005,26(1):23-25. 4 李春喜.生物统计学M .北京:科学出版社,2005.(上接第1311页) J .中药新药与临床药理1995,6(4):41.2 庄毅.药用真菌新型(双向型)固体发酵工程J .中国食用菌,2002,21(4):3-6.3 葛长荣,曹正辉,苏子峰.天然植物提取物在动物生产中的应用及研究 J .饲料添加剂,2005(6):22.4 陈玉库,邢玉娟,赵爱华,等.中草药饲料添加剂在肉鸡生产中的应用 J .动物生产,2006,42(7):62.5 宋学省.中草药添加剂 J .安全饲料,2006,20(8):43. 6 兰进,杨峻山,徐锦堂.抗肿瘤药用真菌资源及利用 J .中药材,1999,22(12):614-618.7 章克昌.药用真菌研究开发的现状及其发展J .食品与生物技术,2001,10(15)99-103. 8 张娟, 路金才.皂苷的提取方法及含量测定研究进展 J .中国现代中药,2006,8(3):26-28.9 唐军.比色法测定黄芪中黄芪总皂苷含量 J .安徽中医学院学报,2004,23(5):37-38. 10 黄俊斌.黄芪注射液中黄芪总皂苷的含量测定 J .时珍国医国药,2001,12(5):394-395.131336卷4期李娜褐菇多糖提取工艺的优化