103?jVol.10,No.32003M9Journal of Tianjin Agricultural College September, 2003cI|:1008-5394(2003)03-0030-04m(4| 刘金福,张平平,赵鑫(?jJS,?300384)K1:m(4|。|1、4、4HWY?i4Iy,Lm(4|HqL。LTV:1120,4100 ,4HW1 h,Y?i85%KD4|Hq;m(Y4|q7m(3.02%,c28.6%。1oM:m(;4|ms|:S642.5;TS218;TS255.5DSM:AStudy on Extraction Techniques of Polysaccharide fromMomordica charantialLIUJin-fu, ZHANGPing-ping, ZHAO Xin(Department of Food Science ,Tianjin AgriculturalCollege, Tianjin 300384, China)Abstract:The extraction techniques of polysaccharide from Momordicacharantial was studied.The ratio of raw material towater, extraction temperature, time and ethanol concentration for precipitation were selected as four factors to design the or-thogonal test and theoptimum condition of extraction techniques was obtained.Theresults show the optimum condition for theextraction technique is as following:ratio of raw material to water is 120, extracting temperatureis 100 , timeis 1 h, con-centration of ethanol forprecipitation is 85%.Theextraction ratioof rudepolysaccharidefrom fresh Momordicacharantial is 3.02%and the polysaccharide content is 28.6% in the raw products.Key words:Momordicacharantial ;water-soluble polysaccharide;extractionm(RSm(,(J+,3Bv,H9?J。m(me,、=、TL0W(0:,Ab、)!、mr 1 。?C,m(、m(4|F)、48f、F?、)hHIVVT 2 。S=Vu、m(#0s3,m(cm(charantin)sAT 3 。M,#3STM,#/?Z,s+l,S=Sj3S-5,.421WW 4 。f,?9(1961-),3,?g,q,v,Z_JS。:(022)23782596。3,:m(4|31,1/,m(?。1ZE1.1!m(:gr,1?gbu。1k4:Y?、d、Y(B)、D-$、i;(sB)、YY(sB)、x1N!:x、br、2Q、b2Q、?、Q、F。1.2LZE1.2.14|7m(M2xY?4VrbAiA?!2m(1.2.2ZEx-;E 7 。1.2.3T11.2.3.1)7m(!、M,b2Q=2,2Q=e60 /,sM。2m(x,V40,m(x,LPm(s$4。1.2.3.2Y?|200 gm(x,500 mL 80%Y?4|2Q,Q2 h 。qS、 8 。rARC RD RA ,:YY4|qLyQ:B(4)、C(4HW)、D(Y?i)、A(1)。yY4|qYsY:B B B ,C C C ,D D D , A A A 。yN,Y4|qKqBCD A ,4100 ,4|HW1 h,HY?i75%,1120。3,:m(4|33m1y4|qYm2ycYRvlRBRCRDRA,YYcLyQ:B(4)、C(4HW)、D(1)、A(Y?i)。ycYsY:B B B , C C C ,D D D , A A A 。yN,YcKqBCDA 。4100 ,4|HW1 h,HY?i85%,1120。2.3s)m(x,4、Y?ZE4|,4|qcTIS,!4Iy,VkTA,4y2SYQB,BCDA。ts。4(B)Y4|qK1y。BS=,4|P%C7P 12 。VQk!939VA,6,4|qc(9F,VRVA,WYA。4|100 H,KD4|。,100 4|QL!9K,B44|,4|qV?4,V4|3,yN,4|B100 /。,100 !1p,?hv,y73Fv。4HW(C)=Yy。!34HW,HW1 hz。qS、P4|H,HW(B3、4 h)4 13,14 。LV,HWm(4aR。(4|H9B 15 。94Tv4|HiHW。Y?i(D)4|qcYi7,Wl。B8Y?rBiH(47%)7SCC,Y?