DC Detection Method,The Pulse,First Dilution 1:500 (WBC) Second Dilution 1:50.000 (RBC),Sample Beaker DB - 1,Dilutions for Semiautomatic Analyser,DC Detection Method,The Pulse,WBC Dilution + 3 Drops Quicklyser for Lysing and HGB,RBC Dilution,Dilutions for Semiautomatic Analyser,DC Detection Method,The Pulse,External Electrode,Aperture,Internal Electrode,DC Detection Method,Transducer,Vacuum,Blood cells,External Electrode,DC Detection Method,Transducer,External Electrode,Aperture,Internal Electrode,DC Detection Method,External Electrode,Aperture,Internal Electrode,U = R x I,The Pulse,DC Detection Method,U = R x I,External Electrode,Aperture,Internal Electrode,The Pulse,DC Detection Method,Time sec,Blood suspension,Transducer,Start-Sensor,Stop-Sensor,Manometer,Counting Time,(defined Volume),forerun,afterrun,Absolute Counting I,Time sec,Blood suspension,Transducer,Start-Sensor,Stop-Sensor,Manometer,Counting Time,(defined Volume),Prerun,Afterrun,DC Detection Method,Absolute Counting I,Time sec,Blood suspension,Transducer,Start-Sensor,Stop-Sensor,Manometer,Counting Time,(defined Volume),Prerun,Afterrun,DC Detection Method,Absolute Counting I,Time sec,Blood suspension,Transducer,Start-Sensor,Stop-Sensor,Manometer,Counting Time,(defined Volume),Prerun,Afterrun,DC Detection Method,Absolute Counting I,Time sec,Blood suspension,Transducer,Start-Sensor,Stop-Sensor,Manometer,Counting Time,(defined Volume),Prerun,Afterrrun,DC Detection Method,Absolute Counting I,DC Detection Method,Absolute Counting,DC Detection Method,Absolute Counting II,Not to be changed by User: Sample Volume Dilution Ratio Counted Volume Aperture Diameter Automatically checked by the instrument: Counting Time Condition of aperture during measurement (Noise-Control) Advantages: No calibration of counters Automatic check of aperture,DC Detection Method,Relative Counting,DC Detection Method,Measurement of particles in a defined volume Calculation of cell-concentration with known Sample volume and defined Sample dilution no calibration of counting values,Measurement of particles per time period The count is received indirectly through a reference-sample. It is taken for granted that the sample and the reference-sample have the same characteristics and give the same result. Calibration of counting values in necessary,Absolute Counting,Relative Counting,Comparison of Absolute and Relative Counting,Pulse Height,Volume (Time),DC Detection Method,1,2,3,4,5,6,7,8,9,10,11,12,13,14,Cumulative Size Distribution Curve,Pulse Picture,10,20,30,1,2,3,4,5,6,7,8,9,10,11,12,13,14,4,1,0,0,0,1,2,3,4,5,4,3,2,1,DC Detection Method,Histogram,1,2,3,4,5,6,7,8,9,10,11,12,13,14,Histogram,4,1,0,0,0,1,2,3,4,5,4,3,2,1,Cumulative Size Distribution Curve,10,20,30,1,2,3,4,5,6,7,8,9,10,11,12,13,14,4,1,0,0,0,1,2,3,4,5,4,3,2,1,DC Detection Method,Histogram,Erythrocyte Histogram,25-75 fl,200-250 fl,DC Detection Method,Histogram,Platelet Histogram,2-6 fl,12-30 fl,fixed at 12 fl,DC Detection Method,Histogram,DC Detection Method,Histogram,Operation Sequences,Histogram,All solenoid valves are de-energized. C (Common) and NO (Normally Open) solenoid valve ports are connected. Ball Float is in the down position.,“READY”-Status,Operation Sequences,Histogram,COUNT KEY on,SV1 and SV 4 are energized. C and NC solenoid valve ports are connected. Diluent is aspirated through SV4, SV2, Transducer, SV1 and SV3. The Ball Float in the manometer is pushed upward. The inner portion of transducer and manometer is automatically rinsed to minimize carry-over effect.,Operation Sequences,Histogram,Counting,SV1 and SV4 are de-energized and SV2 is energized. The diluted sample is aspirated through the aperture and the Ball Float moves downward. When the Ball Float reaches level A, the counter starts counting. When the Ball Float reaches level B, the counting stops and SV2 is de-energized. The volume of aspirated sample is the same as the manometer-volume between points A and B.,Operation Sequences,Histogram,SV1 and SV3 are energized. Pressure Pump is activated. Pressure is applied to the aperture to remove the clog. The same function occurs if the FLUSH key is pressed.,Clog removal,Operation Sequences,Histogram,When power switch is turned on, SV1 and SV4 are energized. Diluent is aspirated through the transducer. The same function occurs if the FILL key is pressed. The diluent removes diluent and any blood sample remaining in the system.,Diluent Fill,Haemoglobin,Histogram,Haemoglobin is the dye at the RBC. It is a Fe-containing Protein. Haemoglobin is exclusively produced by Erythroblasts in the