Chapter 10 Recombination / 重组,10.1 Homologous Recombination 10.1.1 Mechanism for Crossing-Over 10.1.2 Mechanism for Double- Stranded Break Repair 10.1.3 The RecBCD pathway 10.1.4 Gene Conversion 10.2 Non-homologous Recombination 10.2.1 Transposons 10.2.2 Retrotransposons 10.2.3 Bacteriophage integration,10.1 同源重组 10.1.1 交换机理 10.1.2 双链断裂 修复机理 10.1.3 RecBCD途径 10.1.4 基因转换 10.2 非同源重组 10.2.1 转座子 10.2.2 反转录转座子 10.2.3 噬菌体的整合,10.1 Homologous Recombination 同源重组,Diploid (2n) 2 copies of each chromosome,Homologous Recombination,If these chromosomes were left unchanged ,Child,Grand- child,Identical,Crossing-over in meiosis,Child,Grand- child,Different,Homologous Recombination in DNA repair,Homologous chromosomes,10.1.1 Mechanism for Crossing-Over / 交换机理,Mechanism of recombination in yeast (1/2),Mechanism of recombination in yeast (2/2),Branch migration of Holliday junctions Holliday交叉的分支迁移,Resolution of the Holliday junction Holliday交叉的拆分,Resolution of the Holliday junction to create a crossover 产生交换的Holliday交叉拆分 Resolution of Holliday junctions for non-crossover 不产生交换的Holliday交叉拆分,Resolution of the Holliday junction to create a crossover 产生交换的Holliday交叉拆分,10.1.2 Mechanism for Double-Stranded Break Repair / 双链断裂修复机理,Resolution of Holliday junctions for non-crossover 不产生交换的Holliday交叉拆分,Brca1/Brca2 deletion and breast cancer Brca1/Brca2缺失与乳腺癌,www.harthosp.org/cancer/breast.htm,The roles of BRCA1 and BRCA2 in DNA repair BRCA1和BRCA2在DNA修复中的作用,www-ermm.cbcu.cam.ac.uk/01003118h.htm,Now showing Meselson-Radding Heteroduplex Model,File: biophoto7 Introduction to Genetic Analysis Meselson-Radding heteroduplex Model,10.1.3 The RecBCD Pathway (1/3),The RecBCD Pathway (2/3),The RecBCD Pathway (3/3),10.1.4 Gene Conversion / 基因转换,G T,A T,G C,A T,Homologous, Not identical.,3 5,3 5,3 5,3 5,Gene Conversion / 基因转换,Gene Conversion / 基因转换,Neurospora crassa 粗糙脉孢菌,+,+,m,m,Meiosis I 减数分裂I,Meiosis II 减数分裂II,Mitosis 有丝分裂,+,+,m,m,+,+,m,m,m,m,m,m,+,+,+,+,4 : 4,Neurospora crassa 粗糙脉孢菌,www.nigms.nih.gov/Initiatives/Models/,www.neurosporagenome.org/,www.neurosporagenome.org/,4 : 4,5 : 3,6 : 2,Now showing Gene Conversion,File: biophoto7 Introduction to Genetic Analysis Genetic Consequences of Meselson-Radding Model,10.2 Non-homologous Recombination 非同源重组,10.2.1 Transposons 转座子 10.2.2 Retrotransposons 反转录转座子 10.2.3 Bacteriophage integration 噬菌体的整合,10.2.1 Transposons / 转座子,Transposition / 转座,Generation of direct repeats 同向重复序列的产生,Now showing Replicative transposition,File: biophoto7 Introduction to Genetic Analysis Replicative transposition. (5),10.2.2 Retrotransposons 反转录转座子,LTR-Retrotransposons LTR反转录转座子 Non-LTR Retrotransposons 非LTR反转录转座子,LTR-Retrotransposons LTR反转录转座子,Non-LTR retrotransposons 非LTR反转录转座子,Weavers Molecular Biology, 3rd Edition, Figure 23.31,10.2.3 Bacteriophage integration 噬菌体的整合,Lysis,Lysogeny,Phage integration and excision,attP: attachment sites of phage attB: attachment sites of bacterium,