0.01M TBS,PH7.5(配法：1L双蒸馏水中加入8.5克氯化钠，1.2克Tris和0.45-0.5ml纯乙酸。)从Abcam上获得的配方如下：Tris-HCl buffer： 20 mM Tris-HCl, pH 7.5TBS 10x (concentrated TBS)24.23 g Trizma HCl80.06 g NaClMix in 800 ml ultra pure water.pH to 7.6 with pure HCl.Top up to 1 L.