AS 5013.24.22009iAS 5013.24.22009Australian StandardFood microbiologyMethod 24.2: Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenesEnumeration method(ISO 11290-2:1998, MOD)PREFACEThis Standard was prepared by the Standards Australia Committee FT-024, Food Products, and Constituted Subcommittee FT-024-01, Food Microbiology, to supersede in part AS/NZS 1766.2.151998, Food microbiology, Method 2.15: Examination of specific organismsListeria monocytogenes in dairy products and AS/NZS 1766.2.16.11998, Food microbiology, Method 2.16.1: Examination for specific organismsFood and animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenesDetection method.This Standard is an adoption with national modifications and reproduced from ISO 11290-2:1998, Microbiology of food and animal feeding stuffsHorizontal method for the detection and enumeration of Listeria monocytogenes, Part 2: Enumeration method, including Amendment 1: 2004 which is added at the end of the ISO text. ISO 11290-2:1998 was confirmed on 20 June 2005.Additional requirements for Australian conditions are listed in Appendix ZZ.The objective of this revision is to establish a horizontal method for the enumeration ofListeria monocytogenes.As this Standard is reproduced from an International Standard, the following applies:(a) Its number appears on the cover and title page while the International Standard number appears only on the cover.(b) In the source text this part of ISO 11290 should read this Australian Standard. (c)A full point substitutes for a comma when referring to a decimal marker.(d) Substitute mL for ml whenever it appears.References to International Standards should be replaced by references to equivalentAustralian Standards as follows:www.standards.org.auiiiReference to International Standard Australian StandardISOAS6887Microbiology of food and animal feeding stuffsPreparation of test samples, initial suspension and decimal dilutions for microbiological examination6887-1 Part 1: General rules for thepreparation of the initial suspension and of decimal dilutions7218Microbiology of food and animal feeding stuffsGeneral rules for microbiological examination11290Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenes11290-1 Part 1: Detection method5013Food microbiology5013.11.1Method 11.1: Microbiology of food animal feeding stuffPreparation of test samples initial suspension and decimal dilutions for microbiological examinationGeneral rules for the preparation of the initial suspension and decimal dilutions5013.14Method 14: Microbiology of food and animal feeding stuffsGeneral rules for microbiological examinations5013.24.1Method 24.1: Microbiology of food and animal feeding stuffs Horizontal method for the detection and enumeration of Listeria monocytogenesDetection method (ISO 11290-1:1996, MOD)The laboratory should have a clearly defined quality control system to ensure that the apparatus, culture media, reagents and technique are suitable for the test. The use of positive controls is part of this system.The Standard is downloaded from www.bzfxw.com Standard SharingThe terms normative and informative have been used in this Standard to define the application of the annex or appendix to which they apply. A normative annex or appendix is an integral part of a Standard, whereas an informative annex or appendix is only for information and guidance.INTRODUCTIONBecause of the large variety of food and feed products, this horizontal method may not be appropriate in every detail for certain products. In this case, different methods, which are specific to these products may be used if absolutely necessary for justified technical reasons. Nevertheless, every attempt should be made to apply this horizontal method as far as possible.When this part of ISO 11290 is next reviewed, account will be taken of all information then available regarding the extent to which this horizontal method has been followed and the reasons for deviations from this method in the case of particular products.The harmonization of test methods cannot be immediate, and for certain groups of products International Standards and/or national standards may already exist that do not comply with this horizontal method. It is hoped that when such standards are reviewed they will be changed to comply with this part of ISO 11290 so that eventually the only remaining departures from this horizontal method wil