标准曲线的绘制及曲线方程的计算标准曲线的吸光度测量数据吸光度.、工1-1试官1试管2试官3试管4、一F- 试官5、一M厂试官6第一次0.9091.0731.1451.2371.1921.253第二次0.9041.0311.1361.1781.2841.313第三次0.9291.0541.1051.1351.1441.208平均值0.9141.0531.1291.1831.2071.258标准曲线中的x、y值:蛋白质浓度mg/ml (x)00.020.040.060.080.1吸光度平均 值(y)0.9141.0531.1291.1831.2071.258标准曲线由曲线可知，x、y的关系为回归直线关系，故设直线回归方程为宁=a+bx贝 0 元=x / n =0.05y = y / n =1.124SS x= Ex 2 ( x ) 2/n=0.007SP xy= Exy (Ex)(y)/n=0.02236b= SP xy/ SS x=3.194a= y bx =0.9643所以直线回归方程为y = 0.9643 + 3.194x