MagnetofectionTM（PolyMAG，CombiMAG）- 省钱、省时、高效、简便的新型培养细胞转染试剂 优点：1. 与标准转染方法相比，转染率更高，即被转染细胞的百分比提高。2. 短时间孵育后，与标准转染方法相比，转基因表达提高几千倍。3. 极低量的载体即可获得高转染率和高水平转基因表达，节省昂贵的转染试剂。4. 极大缩短实验时间，基因载体和细胞孵育几分钟即足以获得高转染率，而标准转染需要几小时。5. 可用于转染原代细胞及其他难以转染的细胞6. 适用于DNA、RNA、siRNA、反义寡核苷酸、腺病毒、反录病毒的转染（参看下文表格，选用相应产品）新颖的技术： 利用磁力将结合在磁性纳米颗粒上的基因载体带入靶细胞。MagnetofectionTM 转染培养细胞的产品：PolyMAG：应用广泛的磁性颗粒，一步法与欲转染的核酸混合，成功用于质粒DNA、反义寡核苷酸和siRNA。CombiMAG：可与市场上任何转染试剂（如聚阳离子及脂）共同使用，可与质粒DNA、反义寡核苷酸、siRNA和病毒（如腺病毒、反录病毒载体）结合。适用的细胞：已试验过的有HeLa、HEK293、CHO、NIH 3T3近百种贴壁细胞株，几种悬浮细胞，几十种原代细胞。参看Chemicell公司产品宣传册中的表格。未列出的细胞类型不代表不可使用本产品。磁板：MagnetoFACTOR-24和MagnetoFACTOR-96磁板分别与大多数市场上的24孔和96孔培养板匹配，同时也适用于T-75，6孔及12孔培养板，更大的培养板上被转染细胞的密度不均匀。转染效果举例： MagnetoFACTOR 磁板与大部分生产厂家的多孔板匹配。9001参考价格：￥18759004参考价格：￥9759008参考价格：￥6900（减价促销）购买上述产品详情请联系北京启维益成科技有限公司北京启维益成科技有限公司 Beijing Chief-East Tech Co.,Ltd (Qwbio) 办公时间: 周一至周五8: 30 - 17: 00 全国免费电话:400 810 0506 电话: 010-59870940 59870941传真: 010-59870942 邮箱:qwbioyahoo.com.cn 网址:http:/www.bioconsumable.com http:/www.qwbio.com 地址: 北京市海淀区清河清缘西里2号楼505室 邮编: 100192 Address: Room 505,Building 2,QingYuanXiLi,QingHe,HaiDian District,BeiJing 100192,China部分参考文献：Francois M., Andrea Caprini, Brett Hosking, Fabrizio Orsenigo, Dagmar Wilhelm, CatherineBrowne, Karri Paavonen, Tara Karnezis, Ramin Shayan, Meredith Downes, Tara Davidson,Desmond Tutt, Kathryn S. E. Cheah, Steven A. Stacker, George E. O. Muscat, Marc G. Achen,Elisabetta Dejana & Peter Koopman. Sox18 induces development of the lymphaticvasculature in mice NATURE 2008; 456: 643-648.Guillaud L., Wong R. and Hirokawa N. Disruption of KIF17Mint1 interaction byCaMKIIdependent phosphorylation: a molecular model of kinesincargo release. Nature CellBiology 2008; 10 (1): 19-29.Nakakuki Takashi, Marc R. Birtwistle, Yuko Saeki, NorikoYumoto, Kaori Ide, Takeshi Nagashima,Lutz Brusch, Babatunde A.Ogunnaike, Mariko Okada-Hatakeyama and Boris N. Kholodenko.Ligand-Specific c-Fos Expression Emerges from the Spatiotemporal Control of ErbBNetwork Dynamics. Cell 2010; 141: 1-19.Buerli T., Pellegrino C., Baer K., Lardi-Studler B., Chudotvorova I., Fritschy J-M., Medina I. and Fuhrer C. Efficient transfection of DNA or shRNA vectors intoneurons using Magnetofection NATURE PROTOCOLS 2007; 2(12): 3090-3101.Mykhaylyk O., Antequera Y.S., Vlaskou D. & Plank Ch. Generation of magnetic nonviral genetransfer agents and Magnetofection in vitro. Nature Protocols 2007; 2(10): 1-21.Ufer C., Chi Chiu Wang, Michael F鋒ling, Heike Schiebel, Bernd J. Thiele, E. Ellen Billett, Hartmut Kuhn and Astrid Borchert. Translational regulation of glutathione peroxidase 4 expression through guaninerich sequence-binding factor 1 is essential for embryonic braindevelopment. Genes & Development 2008; 22: 1838-1850.Hofmann A., Wenzel D., Becher U.M., Freitag D.F., Klein A. M., Eberbeck D., Schulte M.,Zimmermann K., Bergemann C., Gleich B., Roell W., Weyh T., Trahms L., Nickenig G.,Fleischmann B. K. and Pfeifer A. Combined targeting of lentiviral vectors and positioning oftransduced cells by magnetic nanoparticles. PNAS 2009; 106 (1): 44-49.McBain S.C., U. Griesenbach, S. Xenariou, A. Keramane, C.D. Batich, E.W.F.W. Alton and J.Dobson. Magnetic nanoparticles as gene delivery agents: enhanced transfection in thepresence of oscillating magnet arrays. Nanotechnology 2008; 19: 405102 (5pp).Plank C., Schillinger U., Scherer F., Bergemann C., Remy J.-S., Kr鰐z F., Anton M., Lausier J. and Rosenecker J. The Magnetofection Method: Using Magnetic Force to Enhance GeneDelivery. Biol. Chem. May 2003; 384: 737-747.Plank C., Scherer F., Schillinger U., Bergemann C., Anton M. Magnetofection: enhancing andtargeting gene delivery with superparamagnetic nanoparticles and magnetic fields. J.Liposome Res. 2003; 13(1): 29-32.Rashmi K. Ambasta, Pravir Kumar et al. Direct Interaction of the Novel Nox Proteins withp22phox Is required for the Formation of a Functionally Active NADPH Oxidase.J. of Biological Chem. 2004; 279 (44): 45935-45941.Basile J. R.,Talayeh Afkhami and J. Silvio Gutkind. Semaphorin 4D/Plexin-B1 InducesEndothelial Cell Migration through the Activation of PYK2, Src, and thePhosphatidylinositol 3-KinaseAkt Pathway. Molecular and Cellular Biology 2005; 25 (16):68896898.Fransen M., Ilse Vastiau, Chantal Brees, Vanessa Brys Guy P. Mannaerts and Paul P. VanVeldhoven. Analysis of Human Pex19ps Domain Structure by Pentapeptide ScanningMutagenesis. J. Mol. Biol. 2005; 346: 12751286.Goulimari P. et al. G12/13 Is Essential for Directed Cell Migration and Localized Rho-Dia1Function. JBC 2005; 280 (51): 42242-42251.Liman J. et al. Interaction of BAG1 and Hsp70 Mediates Neuroprotectivity and Increase