不同内切酶对识别位点以外最少保护碱基数目的要求 不同内切酶对识别位点以外最少保护碱基数目的要求酶寡核苷酸序列切割率2 hr20 hrAcc IGGTCGACCo0CGGTCGACCGo0CCGGTCGACCGGo0Afl IIICACATGTGo0CCACATGTGG9090CCCACATGTGGG9090Asc IGGCGCGCC9090AGGCGCGCCT9090TTGGCGCGCCAA9090Ava ICCCCGGGG5090CCCCCGGGGG9090TCCCCCGGGGGA9090BamH ICGGATCCG1025CGGGATCCCG9090CGCGGATCCGCG9090Bgl IICAGATCTG00GAAGATCTTC7590GGAAGATCTTCC2590BssH IIGGCGCGCC00AGGCGCGCCT00TTGGCGCGCCAA5090BstE IIGGGT(A/T)ACCC010BstX IAACTGCAGAACCAATGCATTGG00AAAACTGCAGCCAATGCATTGGAA2550CTGCAGAACCAATGCATTGGATGCAT2590Cla ICATCGATG00GATCGATC00CCATCGATGG9090CCCATCGATGGG5050EcoR IGGAATTCC9090CGGAATTCCG9090CCGGAATTCCGG9090Hae IIIGGGGCCCC9090AGCGGCCGCT9090TTGCGGCCGCAA9090Hind IIICAAGCTTG00CCAAGCTTGG00CCCAAGCTTGGG1075Kpn IGGGTACCC00GGGGTACCCC9090CGGGGTACCCCG9090Mlu IGACGCGTC00CGACGCGTCG2550Neo ICCCATGGG00CATGCCATGGCATG5075Nde ICCATATGG00CCCATATGGG00CGCCATATGGCG00GGGTTTCATATGAAACCC00GGAATTCCATATGGAATTCC7590GGGAATTCCATATGGAATTCCC7590Nhe IGGCTAGCC00CGGCTAGCCG1025CTAGCTAGCTAG1050酶寡核苷酸序列切割率2 hr20 hrNot ITTGCGGCCGCAA00ATTTGCGGCCGCTTTA1010AAATATGCGGCCGCTATAAA1010ATAAGAATGCGGCCGCTAAACTAT2590AAGGAAAAAAGCGGCCGCAAAAGGAAAA2590Nsi ITGCATGCATGCA1090CCAATGCATTGGTTCTGCAGTT9090Pae ITTAATTAA00GTTAATTAAC025CCTTAATTAAGG090Pme IGTTTAAAC00GGTTTAAACC025GGGTTTAAACCC050AGCTTTGTTTAAACGGCGCGCCGG7590Pst IGCTGCAGC00TGCACTGCAGTGCA1010AACTGCAGAACCAATGCATTGG9090AAAACTGCAGCCAATGCATTGGAA9090CTGCAGAACCAATGCATTGGATGCAT00Pvu ICCGATCGG00ATCGATCGAT1025TCGCGATCGCGA010Sac ICGAGCTCG1010Sac IIGCCGCGGC00TCCCCGCGGGGA5090Sal IGTCGACGTCAAAAGGCCATAGCGGCCGC00GCGTCGACGTCTTGGCCATAGCGGCCGCG1050G1075ACGCGTCGACGTCGGCCATAGCGGCCGCGGAASea IGAGTACTC1025AAAAGTACTTTT7575Sma ICCCGGG010CCCCGGGG010CCCCCGGGGG1050TCCCCCGGGGGA9090Spe IGACTAGTC1090GGACTAGTCC1090CGGACTAGTCCG050CTAGACTAGTCTAG050Sph IGGCATGCC00CATGCATGCATG025ACATGCATGCATGT1050Stu IAAGGCCTT9090GAAGGCCTTC9090AAAAGGCCTTTT9090Xba ICTCTAGAG00GCTCTAGAGC9090TGCTCTAGAGCA7590CTAGTCTAGACTAG7590Xho ICCTCGAGG00CCCTCGAGGG1025CCGCTCGAGCGG1075Xma ICCCCGGGG00CCCCCGGGGG2575CCCCCCGGGGGG5090TCCCCCCGGGGGGA9090