溶液成分不同体积（ml ）凝胶液中各成分所需体积（ml ）5101520253040506% SDS-PAGE水2.65.37.910.613.215.921.226.530%丙烯酰胺溶液12345681010% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048% SDS-PAGE水2.34.66.99.311.513.918.523.230%丙烯酰胺溶液1.32.745.36.7810.713.31.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0030.0060.0090.0120.0150.0180.0240.0310% SDS-PAGE水1.945.97.99.911.915.919.830%丙烯酰胺溶液1.73.356.78.31013.316.71.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0212% SDS-PAGE水1.63.34.96.68.29.913.216.530%丙烯酰胺溶液2468101216201.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.0215% SDS-PAGE水1.12.33.44.65.76.99.211.530%丙烯酰胺溶液2.557.51012.51520251.5 mol/L Tris (pH8.8)1.32.53.856.37.51012.510% SDS0.050.10.150.20.250.30.40.510%过硫酸氨0.050.10.150.20.250.30.40.5TEMED0.0020.0040.0060.0080.010.0120.0160.02