2013 Waters Corporation 12013 Waters Corporation 2Waters UPLC Troubleshooting Training 2013 Waters Corporation 3AgendaAgendaACQUITY UPLC Family ：UPLC & H-ClassWaters UPLC SystemUPLC Solvent Management (BSM)UPLC Sample Management (SM)Waters ACQUITY H-Class SystemQuaternary Solvent Manager (QSM) Sample Manager-Flow Through Needle (SM-FTN)Waters UPLC Detector Troubleshooting TrainingWaters UPLC PDA and PDAeTUVFLRELSDOpen Discussion2013 Waters Corporation 4ACQUITY UPLC FamilyACQUITY UPLC Family2013 Waters Corporation 5ACQUITY UPLC SystemACQUITY UPLC System2013 Waters Corporation 6ACQUITY UPLC SystemACQUITY UPLC SystemDetectors:Optical and/or Mass SpecTunable UV or Photodiode ArrayOptimized flow cell for UPLCHigh speed data samplingColumn Manager:Innovative pivot designPositions column to detectorE-Cord connectionSample Manager:Low dispersion XYZZ FormatFast cycle timesLow carryoverPlates and/or vialsOptional Sample OrganizerBinary Solvent Manager:High pressure blendingBinary gradientsFour solvent choicesOn-line degassingLow dispersion designUPLC pressure capabilitiesSample Organizer: (option)Expands capacity (22/15/8)Shuttles plate feedHeated/chilledSystem Considerations:Small FootprintRedesigned tubing and fittingsConsolidated waste managementIntegrated system diagnosticsConnections Insight remote diagnostics2013 Waters Corporation 7A1 A1 A2A2B1B1B2B2WasteFluidics Fluidics and Connectionsand ConnectionsTubing management Chromatographic solvents:oA1/A2, B1/B2Two Sample Manager wash solventsSeal WashCommon fittings and tools for usersColumn inlets/outlets and fittings oFinger tight, turn (use 1/4” wrench)Dedicated tubing3 piece compression screw and ferruleSmall footprint 13” wide24.25” with Sample Organizer2013 Waters Corporation 8UPLCSolvent Manager2013 Waters Corporation 9ACQUITY UPLC System ACQUITY UPLC System Solvent DeliverySolvent Delivery 15 K psi performanceAccurate and precise flow ratesAccurate and precise compositionLow delay volume for gradient applicationso5% Ethyl AcetateToluene Chlorinated solvents: (Trichlorobenzene) 2013 Waters Corporation 15UPLCSample Manager2013 Waters Corporation 16Sample Manager Fluidics TraySample Manager Fluidics Tray Pressure Transducer Valve Manifold Drive Gears Wash SyringesWeak Wash Strong WashMetering Syringe Drive Assembly 2013 Waters Corporation 17Metering Syringe Valve3 Way Rotary ValveMetering Syringe AssemblyMetering Syringe AssemblySample Syringe50 uL volume PN 410001348100 L volume PN 700002570250 L volume PN 410001347ofor use with 50 L loop2013 Waters Corporation 18Column Heating CompartmentColumn Heating Compartment2013 Waters Corporation 19ACQUITY In-Line FilterACQUITY In-Line Filter192013 Waters Corporation 20Column In-Line Filter AssemblyColumn In-Line Filter Assembly2013 Waters Corporation 21Column In-Line Filter AssemblyColumn In-Line Filter Assembly2013 Waters Corporation 22Column HeatingColumn Heating2013 Waters Corporation 23Sample Manager Flow PathSample Manager Flow Path2013 Waters Corporation 24Available Loops Available Loops Description P/NStatus1 L Loop430001516Optional2 L Loop430001264Optional5 L Loop430001233Optional10 L Loop430001326Standard20 L Loop430001320Optional 50 L Loop430001325Optional100 L Loop170001792(Kit) Optional250 L Loop176001780(Kit) Optional2013 Waters Corporation 25Volume Configuration MenuVolume Configuration MenuBefore running the Characterize Needle and Loop Volume function, make sure the proper volumes are configured in the Volumes Configuration menu. The above values are standard for currently shipping ACQUITY system.2013 Waters Corporation 26Recommended Injection Volumes,Recommended Injection Volumes,Loop Sizes and Injection ModesLoop Sizes and Injection ModesLoop Volume (uL)Partial Loop w/Needle Overfill Mode Recommended sample injection volume range (uL)Partial Loop (Pressure Assist Mode) Recommended sample injection volume range (uL)Full Loop Mode Recommended sample injection volume (uL0Automatic overfill factor1uL0.2 to 0.75Not Recommended1.05.82uL0.4 to 1.5Not Recommended2.05.65uL1.0 to 3.8Not Recommended5.0510uL2.0 to 7.52.0 to 5.010.0420uL4.0 to 15.04.0 to 10.020.0350uL10.0 to 37.510.0 to 25.050.02100uL20.0 to 75.020.0 to 50.0100.02250uL40.0 to 150.040.0 to 125.0250.01.5Recommended PLNO injection volume 20% to 75% of loop volumeRecommended Partial Loop Injection 20% to 50% of loop volume2013 Waters Corporation 27ACQUITY H-Class SystemACQUITY H-Class SystemQSMQuaternary Solvent Manager SM-FTNSample Manager-Flow Through Needle CH-AHigh Temperature Column HeaterActive Pre-Heating DetectorsTUV, PDA ePDA, FLR, ELSD2013 Waters Corporation 28The ACQUITY H-Class System consists of the following:Quaternary Solvent Manager (QSM)Four solvent low pressure gradient mixing Supports UPLC PressuresLow gradient delay volumeSM-FTN (Sample Manager-Flow Through Needle)One injection mode- direct inject typeMuch simpler- Just specify injection volumeExcellent recovery and precisionCH-AHigh temperature column heater with active pre-heatingACQUITY H-Class SystemACQUITY H-Class System2013 Waters Corporation 29QSM Fluidics-Front ViewQSM Fluidics-Front ViewPrimary HeadAccumulator HeadVent Valve100ul MixerGradient Proportioning Valve PairsLow PressureManifoldDegasser ChambersSeal Wash Pump2013 Waters Corporation 30QSM Console Top Level MenuQSM Console Top Level Menu2013 Waters Corporation 31ACQUITY H-Class SystemACQUITY H-Class System2013 Waters Corporation 322013 Waters Corporation 33SMDI Front Panel FluidicsSMDI Front Panel FluidicsMetering Syringe ValveCheck Valve and UnionInject ValveWash Solvent Inlet Line (not degassed)Leak Sensor ConnectionsSample Metering Syringe2013 Waters Corporation 34SDMI Inject Valve ConnectionsSDMI Inject Valve ConnectionsTo Check ValveTo CH-A and ColumnStationary Side TubeFrom PumpTo Pressure TransducerTo Sample Needle2013 Waters Corporation 35Sample CompartmentSample CompartmentPower and Status LEDsPlate Selector SwitchRotary Tray(Plate Holder)Magnetic Door SensorPower Switch2013 Waters Corporation 36SM-FTN Interactive Display MenuSM-FTN Interactive Display Menu2013 Waters Corporation 37CH-ACH-AImproved Door GasketAdded Tubing Gasket (top and bottom)For 3rd party Mass Spec at left side ofACQUITY Stack.Allows use of existingPassive Pre-heaterNo wires to connectActive Pre-heater2013 Waters Corporation 38Installing the Active Pre-HeaterInstalling the Active Pre-Heater2013 Waters Corporation 39Gold Plated Compression ScrewPEEK FerruleStainless Steel Cap NutStainless Steel Tubing (with brazed on collet)Installing the Active Pre-HeaterInstalling the Active Pre-Heater2013 Waters Corporation 40UPLCDetectors2013 Waters Corporation 41DetectorsDetectorsSupports all current ACQUITY detectorsPDA and PDAeTUVFLRELSD2013 Waters Corporation 42ACQUITYACQUITY UPLCUPLC PDAPDA2013 Waters Corporation 43ACQUITYACQUITY UPLCUPLC PDA PM kitsPDA PM kits2013 Waters Corporation 44ACQUITYACQUITY UPLCUPLC PDAPDA Optics AssemblyOptics Assemblyand Spectrumand Spectrum一类参考2013 Waters Corporation 45ACQUITYACQUITY UPLCUPLC TUVTUV一类参考2013 Waters Corporation 46ACQUITYACQUITY UPLCUPLC TUVTUV Optics AssemblyOptics Assembly一类参考2013 Waters Corporation 47ACQUITYACQUITY UPLCUPLC TUVTUV Optics AssemblyOptics Assembly一类参考2013 Waters Corporation 48ACQUITYACQUITY UPLCUPLC FLRFLR2013 Waters Corporation 49ACQUITYACQUITY UPLCUPLC FLRFLR Optics AssemblyOptics Assembly一类参考2013 Waters Corporation 50ACQUITYACQUITY UPLCUPLC ELSDELSD一类参考2013 Waters Corporation 51Questions?一类参考2013 Waters Corporation 52