1 / 41Chapter 10 Recombination / 重组重组10.1 Homologous Recombination 10.1.1 Mechanism for Crossing-Over 10.1.2 Mechanism for Double- Stranded Break Repair 10.1.3 The RecBCD pathway 10.1.4 Gene Conversion10.2 Non-homologous Recombination 10.2.1 Transposons 10.2.2 Retrotransposons 10.2.3 Bacteriophage integration10.1 同源重组同源重组 10.1.1 交换机理交换机理 10.1.2 双链断裂双链断裂 修复机理修复机理 10.1.3 RecBCD途径途径 10.1.4 基因转换基因转换10.2 非同源重组非同源重组 10.2.1 转座子转座子 10.2.2 反转录转座子反转录转座子 10.2.3 噬菌体的整合噬菌体的整合2 / 4110.1 Homologous Recombination 同源重组同源重组Diploid (2n)2 copies of eachchromosome3 / 41Homologous Recombination4 / 41If these chromosomes were left unchanged ChildGrand-childIdentical5 / 41Crossing-over in meiosisChildGrand-childDifferent6 / 41Homologous Recombination in DNA repairHomologouschromosomes7 / 4110.1.1 Mechanism for Crossing-Over / 交换机理交换机理8 / 41Mechanism of recombination in yeast (1/2)9 / 41Mechanism of recombination in yeast (2/2)Holliday junctions10 / 41Branch migration of Holliday junctionsHolliday交叉的分支迁移交叉的分支迁移11 / 41Resolution of the Holliday junctionHolliday交叉的拆分交叉的拆分Resolution of the Holliday junctionto create a crossover产生交换的产生交换的Holliday交叉拆分交叉拆分Resolution of Holliday junctionsfor non-crossover不产生交换不产生交换的的Holliday交叉拆分交叉拆分12 / 41Resolution of the Holliday junctionto create a crossover产生交换的产生交换的Holliday交叉拆分交叉拆分13 / 4110.1.2 Mechanism for Double-Stranded Break Repair / 双链断裂修复机理双链断裂修复机理14 / 41Resolution of Holliday junctionsfor non-crossover不产生交换的不产生交换的Holliday交叉拆分交叉拆分15 / 41Brca1/Brca2 deletion and breast cancer Brca1/Brca2缺失与乳腺癌缺失与乳腺癌www.harthosp.org/cancer/breast.htm 16 / 41The roles of BRCA1 and BRCA2 in DNA repair BRCA1和和BRCA2在在DNA修复中的作用修复中的作用www-ermm.cbcu.cam.ac.uk/01003118h.htm 17 / 41Now showingMeselson-Radding Heteroduplex ModelFile: biophoto7 Introduction to Genetic Analysis Meselson-Radding heteroduplex Model18 / 4110.1.3 The RecBCD Pathway (1/3)19 / 41The RecBCD Pathway (2/3)20 / 41The RecBCD Pathway (3/3)21 / 4110.1.4 Gene Conversion / 基因转换基因转换G GT TA AT TG GC CA AT THomologous,Not identical.3535353522 / 41Gene Conversion / 基因转换基因转换23 / 41Gene Conversion / 基因转换基因转换24 / 41Neurospora crassa 粗糙脉孢菌粗糙脉孢菌+mmMeiosis I减数分裂减数分裂IMeiosis II减数分裂减数分裂IIMitosis有丝分裂有丝分裂+mm+mmmmmm+4 : 425 / 41Neurospora crassa 粗糙脉孢菌粗糙脉孢菌www.nigms.nih.gov/Initiatives/Models/ www.neurosporagenome.org/ www.neurosporagenome.org/ 4 : 45 : 36 : 226 / 41Now showingGene ConversionFile: biophoto7 Introduction to Genetic Analysis Genetic Consequences of Meselson-Radding Model27 / 4110.2 Non-homologous Recombination非同源重组非同源重组10.2.1 Transposons 转座子转座子10.2.2 Retrotransposons 反转录转座子反转录转座子10.2.3 Bacteriophage integration 噬菌体的整合噬菌体的整合28 / 4110.2.1 Transposons / 转座子转座子29 / 41Transposition / 转座转座31 / 41Generation of direct repeats同向重复序列的产生同向重复序列的产生34 / 41Now showingReplicative transpositionFile: biophoto7 Introduction to Genetic Analysis Replicative transposition. (5)37 / 4110.2.2 Retrotransposons 反转录转座子反转录转座子LTR-RetrotransposonsLTR反转录转座子反转录转座子Non-LTR Retrotransposons非非LTR反转录转座子反转录转座子38 / 41LTR-RetrotransposonsLTR反转录转座子反转录转座子39 / 41Non-LTR retrotransposons非非LTR反转录转座子反转录转座子Weavers Molecular Biology, 3rd Edition, Figure 23.3140 / 4110.2.3 Bacteriophage integration噬菌体的整合噬菌体的整合LysisLysogeny41 / 41Phage integration and excisionattP: attachment sites of phageattB: attachment sites of bacterium