三个纵轴双 Y 轴的曲线图（柱形图）是我们在写学术论文中常常要用到的方法 ,下面是我总结的在origin 中画过双 Y 轴的曲线图（柱形图）的方法（自己摸索的)。1.将数据分三列输入表格中,第一列为 X，其余两列为 Y;2。先选中前两列，画折线图；若画柱形图，双击柱形 ,在对话框中将实心的柱形改为空心的,并在“Spaceing”下将 20改为 80，主要其区别作用。3.选中图形窗口（图形窗口上的标题栏为蓝色） ,右击图形窗口的右侧的空白处， 点击右击菜单中的“NewLayer（Axes)”，在二级菜单中选择“RightY”.此时右侧出现右 Y 轴。同时图形的左上角显示“1，2”,其实是图形的两个层，我们下面就是要将另一个图形画在层2 上.4。回到表格窗口，选中第三列数据，回到图形窗口并注意图形窗口的左上方的 “2按钮选中（一定要点击图形窗口，上面标题栏为蓝色）,点 Graph 菜单下的“AddPlot toLayer选择柱形图，这时，另一个曲线图（或柱形图）就画出来了.其他设置可自行完成。以上方法对画曲线图比较有利,但画柱形图存在在同一个X处图形重叠的问题,我用的办法是步骤1 中的区分方法，不知道各位虫子有没有更好的方法！P-concentrationIAA Concentration Bacterial concentration6018BacterialConcentrationLOG10(CFUml)-1125616P-Concentration（mgL）852121086426484442040010305070Corn Flour Concentration（g/L)IAAconcentration(mgL)-1-11014P-concentrationIAA ConcentrationBacterial concentration2050BacterialConcentrationLOG10(CFUml)-11210818P-Concentration（mgL）14121086423062042051015201000Soybean meal Concentration（g/L)IAAconcentration(mgL)-14016-1-1BacterialconcentrationLOG10(CFUml)IAA ConcentrationP-concentration Bacterial concentration6456121086420SMgPDPSMnSCaFC141210864204032241680Inorganic salts（g/L)-1P-concentration(mgL)48-1IAAConcentration(mgL) BBacterial concentration CIAA concentration DP-concentration9018168886BacterialconcentrationLOG10(CFUml)14.013.513.012.512.0-1IAAconcentration(mgL)-11412108611.512243648607248482807876747270Time(h)P-concentration(mgL)-1Bacterial concentrationP-concentrationIAA concentration14.06059585712.55612.05511.511.010.5243648607284965453522019BacterialconcentrationLOG10(cfumL-1)13.513.0171615141312Time(h)IAAconcentration(mgmL)P-concentration(mgmL)-1-11820Bacterial concentration14.0P-concentration IAA concentration585622BacterialconcentrationLOG10(cfumL-1)13.513.052504812.51812.01646444211.51411.02436486072849612Time(h)IAAconcentration(mgmL)54P-concentration(mgmL)-120-125Bacterial concentrationP-concentration14.0IAA concentration7524BacterialconcentrationLOG10（cfumL）13.5227020-113.06512.56012.05518161411.51211.02436486072849650Time(h)IAAconcentration(mgmL)P-concentration(mgmL)-1-130Bacterial concentrationP-concentration14.0IAA concentration8020BacterialconcentrationLOG10（cfumL）75-17013.065166012.5551412.0243648607284965012Time(h)IAAconcentration(mgmL)P-concentration(mgmL)-1-113.5183514BacterialconcentrationLOG10(cfumL)-1131211 20 25 30 3524364860728496Time(h)不同温度条件下的菌体生长情况807570 20 25 30 35P-concentration(mgmL)-165605550454024364860728496Time(h)不同温度条件下菌体的溶磷量24 20 25 30 3522IAAconcentration(mgmL)-1201816141224364860728496Time(h)不同温度条件下的 IAA 含量14.0BacterialconcentrationLOG10(cfumL)-113.513.012.512.011.511.010.524364860728496 110rpm 140rpm 170rpm 200rpmTime(h)不同转速条件下菌体生长情况6664P-concentrationIAA concentration181614121082436486072P-concentration(mgL)-162605856545250Cultivation time(h)IAAconcentration(mgL)-113.8BacterialconcentrationLOG10(CFUml)-113.613.413.213.012.812.612.412.212.011.811.624364860728496 1% 2% 3% 4%Cultivtion time(h)Design-Expert?SoftwarePhosphate solubilization63.944X1 = A: YMFX2 = B: DPActual FactorsC: K2HPO4 = 2.00D: MnSO4 = 0.2064solubleP(mg/l)60.55753.550Design-Expert?SoftwarePhosphate solubilizationDesign Points63.944X1 = A: YMFX2 = B: DPActual FactorsC: K2HPO4 = 2.00D: MnSO4 = 0.20Design-Expert?SoftwareIAA concentrationDesign Points21.26511.858X1 = A: YMFX2 = B: DPActual FactorsC: K2HPO4 = 2.00D: MnSO4 = 0.2020.00 60.0017.50 55.0015.00 50.0012.50 45.0010.00 40.00 corn flour (g/l)Phosphate solubilization20.0052.470256.746354.608354.608317.50)/lg(lae58.8844mn15.005aebyos12.5061.022556.746354.608356.746352.470210.0040.0045.0050.0055.0060.00corn f lour (g/l)IAA concentration (mg/l)20.0017.50)/lg(lae17.6883mn15.005aeby15.324916.5066os12.5014.143212.961510.0040.0045.0050.0055.0060.00corn f lour (g/l) soybean meal (g/l)Design-Expert?SoftwareIAA concentration21.26511.858IAAconcentration(mg/l)X1 = A: YMFX2 = B: DPActual FactorsC: K2HPO4 = 2.00D: MnSO4 = 0.2018.917.115.313.511.720.0017.5015.0012.50 soybean meal (g/l) 45.0010.00 40.00 50.00 55.00 60.00 corn flour (g/l)IAA concentrationP-concentration22201816148642015202530351210864208070Cell growth1412IAAconcentration(mg/l)Cellgrowthlog10(cfu/ml)106050403020100Temperature()P-concentration(mg/l)Cell growth1412IAA concentration2220187060P-concentrationCellgrowthlog10(cfu/ml)IAAconcentration(mg/l)10864201%2%3%4%1412108642050403020100 Inoculation amountP-concentration(mg/l)16Cell growth1412IAA concentrationP-concentration242220188070IAAconcentration(mg/l)Cellgrowthlog10(cfu/ml)108642070100130160150161412108642050403020100Liquid loading(ml)P-concentration(mg/l)60