Gaoyanimagej的使用(分析荧光图片)ImageJFeaturesn nRuns Everywhere:Runs Everywhere: n nImageJiswritteninJava,whichallowsittorunonLinux,MacOSXandImageJiswritteninJava,whichallowsittorunonLinux,MacOSXandWindows,inboth32-bitand64-bitmodes.Windows,inboth32-bitand64-bitmodes.n nOpen Source:Open Source: n nImageJanditsImageJanditsJavasourcecodeJavasourcecodearefreelyavailableandinthearefreelyavailableandinthepublicdomainpublicdomain. .Nolicenseisrequired.Nolicenseisrequired.n nPlugins:Plugins: n nExtendImageJbydevelopingpluginsusingImageJsbuiltintexteditorandExtendImageJbydevelopingpluginsusingImageJsbuiltintexteditorandJavacompiler.Morethan500pluginsJavacompiler.Morethan500pluginsareavailableareavailable. .n nAnalysis:Analysis: n nMeasurearea,mean,standarddeviation,minandmaxofselectionorentireMeasurearea,mean,standarddeviation,minandmaxofselectionorentireimage.Measurelengthsandangles.Userealworldmeasurementunitssuchasimage.Measurelengthsandangles.Userealworldmeasurementunitssuchasmillimeters.Calibrateusingdensitystandards.Generatehistogramsandpro.millimeters.Calibrateusingdensitystandards.Generatehistogramsandpro.imagej的使用(分析荧光图片)Installationn nLinuxn nMacOSXn nMacOS9n nWindowsimagej的使用(分析荧光图片)Windowshttp:/Download ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64-bit Java 1.6.0_20 (24MB; requires 64-bit Windows) or without Java (3MB). imagej的使用(分析荧光图片)OpenReads an image and displays it in a separate window. Files must be in TIFF, GIF, JPEG, DICOM, BMP, PGM or FITS format. Also opens ImageJ and NIH Image lookup tables (with .lut extension). Additional are supported via plugins installed in the Import submenu. imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Subtract backgroundimagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Merge channelimagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Scalebarn nScalebarsshouldbepresentonallpublication/presentationimages/movies.Itsworthputtingtheminsoonerratherthanlater.Chooseastandardscalebarsizeforallyourimagesifpossibletoavoidconfusion.imagej的使用(分析荧光图片)n nIfyouknowthesizeofafeature(previouslyappliedscalebarforinstance)youcanusethiscommandtoapplyacalibration.n nUsingthelineselectiontool,drawalingalongthelengthofthefeature/scalebar.n nRunthemenucommand“Plugins/Spatial calibration/Set scale”1.n nEnterthedimensionsoftheobject/scalebarinthe“known distance”boxandsettheunitsintheUnit of lengthbox.n nDonotcheckGlobal unlessyouwishallyourimagestohavethiscalibration!ClickOK.n nimagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Analyze Particles and count celln nThiscommandcountsandmeasuresobjectsinbinaryorthresholdedimages.Itworksbyscanningtheimageorselectionuntilitfindstheedgeofanobject.Itthenoutlinestheobjectusingthewandtool,measuresitusingtheMeasurecommand,fillsittomakeitinvisible,thenresumesscanninguntilitreachestheendoftheimageorselection.Presstheesckeytoabortthisprocess.UseImageAdjustThresholdtothresholdanimage.imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Selection and cropimagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)imagej的使用(分析荧光图片)Thankyou!2011-4-192011-4-19imagej的使用(分析荧光图片)